Detection of Indiscriminate Genetic Manipulation in Thoroughbred Racehorses by Targeted Resequencing for Gene-Doping Control

Detection of Indiscriminate Genetic Manipulation in Thoroughbred Racehorses by Targeted Resequencing for Gene-Doping Control

The creation of genetically modified horses is prohibited in horse racing as it falls under the banner of gene doping. In this study, we developed a test to detect gene editing based on amplicon sequencing using next-generation sequencing (NGS). We designed 1012 amplicons to target 52 genes (481 exo...

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Bibliographic Details
Main Authors: Teruaki Tozaki, Aoi Ohnuma, Kotono Nakamura, Kazuki Hano, Masaki Takasu, Yuji Takahashi, Norihisa Tamura, Fumio Sato, Kyo Shimizu, Mio Kikuchi, Taichiro Ishige, Hironaga Kakoi, Kei-ichi Hirota, Natasha A. Hamilton, Shun-ichi Nagata
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Genes
Subjects:
amplicon sequencing
gene doping
gene editing
horse
thoroughbred
Online Access:https://www.mdpi.com/2073-4425/13/9/1589

Internet

https://www.mdpi.com/2073-4425/13/9/1589

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