Characterization of a new bifunctional endo-1,4-β-xylanase/esterase found in the rumen metagenome

Abstract Metagenomic data mining of the Nellore cattle rumen microbiota identified a new bifunctional enzyme, endo-1,4-β-xylanase/esterase, which was subsequently overexpressed in E. coli BL21 (DE3). This enzyme was stable at pH intervals of 5 to 6.5 and temperatures between 30 and 45 °C, and under...

Full description

Bibliographic Details
Main Authors: Gabriella Cavazzini Pavarina, Eliana Gertrudes de Macedo Lemos, Natália Sarmanho Monteiro Lima, João Martins Pizauro Jr.
Format: Article
Language:English
Published: Nature Portfolio 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-89916-8