Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides
This study aimed to investigate the inhibitory effects of Porphyra yezoensis polysaccharides (PYP) and Astragalus polysaccharides (APS) with similar molecular weight (approximately 4 KDa) on high phosphate (Pi)-induced vascular calcification (VC) in rat thoracic aortic smooth muscle cells (A7r5) and...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620303844 |
| _version_ | 1818617847593566208 |
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| author | Fang Huang Jia-Yun Chen Jian-Ming Ouyang |
| author_facet | Fang Huang Jia-Yun Chen Jian-Ming Ouyang |
| author_sort | Fang Huang |
| collection | DOAJ |
| description | This study aimed to investigate the inhibitory effects of Porphyra yezoensis polysaccharides (PYP) and Astragalus polysaccharides (APS) with similar molecular weight (approximately 4 KDa) on high phosphate (Pi)-induced vascular calcification (VC) in rat thoracic aortic smooth muscle cells (A7r5) and to develop drugs that can inhibit VC. After co-incubation with 3.0 mmol/L Pi and different concentrations of PYP or APS, calcium deposition and corresponding biochemical indicators of A7r5 cells were detected. PYP and APS remarkably restricted reactive oxygen species generation, restored mitochondrial membrane potential, reduced excessive apoptosis and autophagy, up-regulated expression of α-smooth muscle actin, suppressed alkaline phosphatase activity, and decreased calcium deposition on the surface of A7r5 cells. At the same concentration, PYP with stronger antioxidant capacity exhibited better inhibitory effect on calcification of A7r5 cells. These results suggest that polysaccharide can inhibit calcification by protecting A7r5 cells from high-Pi induced oxidative damage. |
| first_indexed | 2024-12-16T17:12:12Z |
| format | Article |
| id | doaj.art-74ae34edd1e64f76a2e42fb32132b2f5 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T17:12:12Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-74ae34edd1e64f76a2e42fb32132b2f52022-12-21T22:23:23ZengElsevierJournal of Functional Foods1756-46462020-10-0173104160Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharidesFang Huang0Jia-Yun Chen1Jian-Ming Ouyang2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, ChinaInstitute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, ChinaCorresponding author.; Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, ChinaThis study aimed to investigate the inhibitory effects of Porphyra yezoensis polysaccharides (PYP) and Astragalus polysaccharides (APS) with similar molecular weight (approximately 4 KDa) on high phosphate (Pi)-induced vascular calcification (VC) in rat thoracic aortic smooth muscle cells (A7r5) and to develop drugs that can inhibit VC. After co-incubation with 3.0 mmol/L Pi and different concentrations of PYP or APS, calcium deposition and corresponding biochemical indicators of A7r5 cells were detected. PYP and APS remarkably restricted reactive oxygen species generation, restored mitochondrial membrane potential, reduced excessive apoptosis and autophagy, up-regulated expression of α-smooth muscle actin, suppressed alkaline phosphatase activity, and decreased calcium deposition on the surface of A7r5 cells. At the same concentration, PYP with stronger antioxidant capacity exhibited better inhibitory effect on calcification of A7r5 cells. These results suggest that polysaccharide can inhibit calcification by protecting A7r5 cells from high-Pi induced oxidative damage.http://www.sciencedirect.com/science/article/pii/S1756464620303844Vascular calcificationPlant polysaccharidesAnti-oxidationHigh-phosphate |
| spellingShingle | Fang Huang Jia-Yun Chen Jian-Ming Ouyang Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides Journal of Functional Foods Vascular calcification Plant polysaccharides Anti-oxidation High-phosphate |
| title | Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides |
| title_full | Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides |
| title_fullStr | Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides |
| title_full_unstemmed | Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides |
| title_short | Comparison of the inhibition of high phosphate-induced smooth muscle cell calcification by Porphyra yezoensis and Astragalus polysaccharides |
| title_sort | comparison of the inhibition of high phosphate induced smooth muscle cell calcification by porphyra yezoensis and astragalus polysaccharides |
| topic | Vascular calcification Plant polysaccharides Anti-oxidation High-phosphate |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620303844 |
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