A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis

A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis

Fluorescence lifetime imaging (FLIM) is a powerful imaging modality used to gather fluorescent reporter data independent of intracellular reporter intensity or imaging depth. We applied this technique to image real-time activation of a reporter for the proteolytic enzyme, caspase-3, in response to a...

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Bibliographic Details
Main Authors: Johanna M. Buschhaus, Brock Humphries, Kathryn E. Luker, Gary D. Luker
Format: Article
Language:English
Published: MDPI AG 2018-06-01
Series:Cells
Subjects:
apoptosis
breast cancer
caspase-3
fluorescence lifetime imaging
Förster resonance energy transfer
Online Access:http://www.mdpi.com/2073-4409/7/6/57

Internet

http://www.mdpi.com/2073-4409/7/6/57

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