A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis
Fluorescence lifetime imaging (FLIM) is a powerful imaging modality used to gather fluorescent reporter data independent of intracellular reporter intensity or imaging depth. We applied this technique to image real-time activation of a reporter for the proteolytic enzyme, caspase-3, in response to a...
Main Authors: | Johanna M. Buschhaus, Brock Humphries, Kathryn E. Luker, Gary D. Luker |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-06-01
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Series: | Cells |
Subjects: | |
Online Access: | http://www.mdpi.com/2073-4409/7/6/57 |
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