Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury
[INLINE:1] Traumatic brain injury results in neuronal loss and glial scar formation. Replenishing neurons and eliminating the consequences of glial scar formation are essential for treating traumatic brain injury. Neuronal reprogramming is a promising strategy to convert glial scars to neural tissue...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2024;volume=19;issue=3;spage=629;epage=635;aulast=Liu |
| _version_ | 1827779141970165760 |
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| author | Jingzhou Liu Xin Xin Jiejie Sun Yueyue Fan Xun Zhou Wei Gong Meiyan Yang Zhiping Li Yuli Wang Yang Yang Chunsheng Gao |
| author_facet | Jingzhou Liu Xin Xin Jiejie Sun Yueyue Fan Xun Zhou Wei Gong Meiyan Yang Zhiping Li Yuli Wang Yang Yang Chunsheng Gao |
| author_sort | Jingzhou Liu |
| collection | DOAJ |
| description | [INLINE:1]
Traumatic brain injury results in neuronal loss and glial scar formation. Replenishing neurons and eliminating the consequences of glial scar formation are essential for treating traumatic brain injury. Neuronal reprogramming is a promising strategy to convert glial scars to neural tissue. However, previous studies have reported inconsistent results. In this study, an AAV9P1 vector incorporating an astrocyte-targeting P1 peptide and glial fibrillary acidic protein promoter was used to achieve dual-targeting of astrocytes and the glial scar while minimizing off-target effects. The results demonstrate that AAV9P1 provides high selectivity of astrocytes and reactive astrocytes. Moreover, neuronal reprogramming was induced by downregulating the polypyrimidine tract-binding protein 1 gene via systemic administration of AAV9P1 in a mouse model of traumatic brain injury. In summary, this approach provides an improved gene delivery vehicle to study neuronal programming and evidence of its applications for traumatic brain injury. |
| first_indexed | 2024-03-11T14:45:24Z |
| format | Article |
| id | doaj.art-74c127c88ca04d55866119e84b5e90fc |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-03-11T14:45:24Z |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-74c127c88ca04d55866119e84b5e90fc2023-10-30T11:47:16ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742024-01-0119362963510.4103/1673-5374.380907Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injuryJingzhou LiuXin XinJiejie SunYueyue FanXun ZhouWei GongMeiyan YangZhiping LiYuli WangYang YangChunsheng Gao[INLINE:1] Traumatic brain injury results in neuronal loss and glial scar formation. Replenishing neurons and eliminating the consequences of glial scar formation are essential for treating traumatic brain injury. Neuronal reprogramming is a promising strategy to convert glial scars to neural tissue. However, previous studies have reported inconsistent results. In this study, an AAV9P1 vector incorporating an astrocyte-targeting P1 peptide and glial fibrillary acidic protein promoter was used to achieve dual-targeting of astrocytes and the glial scar while minimizing off-target effects. The results demonstrate that AAV9P1 provides high selectivity of astrocytes and reactive astrocytes. Moreover, neuronal reprogramming was induced by downregulating the polypyrimidine tract-binding protein 1 gene via systemic administration of AAV9P1 in a mouse model of traumatic brain injury. In summary, this approach provides an improved gene delivery vehicle to study neuronal programming and evidence of its applications for traumatic brain injury.http://www.nrronline.org/article.asp?issn=1673-5374;year=2024;volume=19;issue=3;spage=629;epage=635;aulast=Liuaav9p1; astrocytes; astrocyte-to-neuron conversion; gfap promoter; glial scar; induced neurons; neuronal reprogramming; p1 peptide; ptbp1; traumatic brain injury |
| spellingShingle | Jingzhou Liu Xin Xin Jiejie Sun Yueyue Fan Xun Zhou Wei Gong Meiyan Yang Zhiping Li Yuli Wang Yang Yang Chunsheng Gao Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury Neural Regeneration Research aav9p1; astrocytes; astrocyte-to-neuron conversion; gfap promoter; glial scar; induced neurons; neuronal reprogramming; p1 peptide; ptbp1; traumatic brain injury |
| title | Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury |
| title_full | Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury |
| title_fullStr | Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury |
| title_full_unstemmed | Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury |
| title_short | Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury |
| title_sort | dual targeting aav9p1 mediated neuronal reprogramming in a mouse model of traumatic brain injury |
| topic | aav9p1; astrocytes; astrocyte-to-neuron conversion; gfap promoter; glial scar; induced neurons; neuronal reprogramming; p1 peptide; ptbp1; traumatic brain injury |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2024;volume=19;issue=3;spage=629;epage=635;aulast=Liu |
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