Protocol for culturing and imaging of ectodermal cells from Xenopus

Summary: The Xenopus embryo provides an advantageous model system where genes can be readily transplanted as DNA or mRNA or depleted with antisense techniques. Here, we present a protocol to culture and image the cell biological properties of explanted Xenopus cap cells in tissue culture. We illustr...

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Bibliographic Details
Main Authors: Nydia Tejeda-Muñoz, Julia Monka, Edward M. De Robertis
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003355