Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics
The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplement...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342030613X |
| _version_ | 1819113079097524224 |
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| author | Nurshahieda Mohamad Hidayah Manan Mhd Sallehhuddin Najiah Musa Mhd Ikhwanuddin |
| author_facet | Nurshahieda Mohamad Hidayah Manan Mhd Sallehhuddin Najiah Musa Mhd Ikhwanuddin |
| author_sort | Nurshahieda Mohamad |
| collection | DOAJ |
| description | The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplemented with 2% of CaCO3 (w/v). Bacterial colonies with strong clear zone were selected for further tests. From these, 17 isolates of LAB showed non-spore forming, homofermentative characteristics, Gram-positive, rod-shaped, non-motile bacteria and absences of catalase enzymes. The LAB was further identified using API 50 CH kit and 16S rRNA. The isolates LAB also showed tolerance to a variety of environmental conditions like pH, temperature, salt, bile and hemolysis activity in in vitro condition. Based on the results, two isolates LAB were identified as Lactobacillus plantarum and Entercoccus faecalis were suggested as potential probiotic candidates from giant freshwater prawn for Aquaculture industries. |
| first_indexed | 2024-12-22T04:23:42Z |
| format | Article |
| id | doaj.art-74e074ea2f504b09a4e030dea7b1bbd2 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-22T04:23:42Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-74e074ea2f504b09a4e030dea7b1bbd22022-12-21T18:39:13ZengElsevierAquaculture Reports2352-51342020-11-0118100523Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probioticsNurshahieda Mohamad0Hidayah Manan1Mhd Sallehhuddin2Najiah Musa3Mhd Ikhwanuddin4Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaInstitute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaInstitute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaFaculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, MalaysiaInstitute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Guangdong 515063, China; Corresponding author.The present study aims to isolate, identify, and evaluate potential probiotic, Lactic Acid Bacteria (LAB) from the gut and hepatopancreas of 10 healthy wild giant freshwater prawn, Macrobrachium rosenbergii. The samples were cultivated using a modified De Man, Rogosa and Sharpe (MRS) agar supplemented with 2% of CaCO3 (w/v). Bacterial colonies with strong clear zone were selected for further tests. From these, 17 isolates of LAB showed non-spore forming, homofermentative characteristics, Gram-positive, rod-shaped, non-motile bacteria and absences of catalase enzymes. The LAB was further identified using API 50 CH kit and 16S rRNA. The isolates LAB also showed tolerance to a variety of environmental conditions like pH, temperature, salt, bile and hemolysis activity in in vitro condition. Based on the results, two isolates LAB were identified as Lactobacillus plantarum and Entercoccus faecalis were suggested as potential probiotic candidates from giant freshwater prawn for Aquaculture industries.http://www.sciencedirect.com/science/article/pii/S235251342030613XTurbotRearing temperaturescFOSGut microbiotaDigestive enzymeGut histology |
| spellingShingle | Nurshahieda Mohamad Hidayah Manan Mhd Sallehhuddin Najiah Musa Mhd Ikhwanuddin Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics Aquaculture Reports Turbot Rearing temperature scFOS Gut microbiota Digestive enzyme Gut histology |
| title | Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics |
| title_full | Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics |
| title_fullStr | Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics |
| title_full_unstemmed | Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics |
| title_short | Screening of Lactic Acid Bacteria isolated from giant freshwater prawn (Macrobrachium rosenbergii) as potential probiotics |
| title_sort | screening of lactic acid bacteria isolated from giant freshwater prawn macrobrachium rosenbergii as potential probiotics |
| topic | Turbot Rearing temperature scFOS Gut microbiota Digestive enzyme Gut histology |
| url | http://www.sciencedirect.com/science/article/pii/S235251342030613X |
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