Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate
The production of polyunsaturated fatty acids from fish oil, which is related to various health benefits including effects against cardiovascular diseases, antihypertensive, anticancer, antioxidant, antidepression, anti-aging, and anti-arthritis effects, among others, can be advantageously performed...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2073-4344/13/2/289 |
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| author | Aparecida Selsiane Sousa Carvalho Júlio Cesar Soares Sales Felipe Valle do Nascimento Bernardo Dias Ribeiro Carlos Eduardo Conceição de Souza Ailton Cesar Lemes Maria Alice Zarur Coelho |
| author_facet | Aparecida Selsiane Sousa Carvalho Júlio Cesar Soares Sales Felipe Valle do Nascimento Bernardo Dias Ribeiro Carlos Eduardo Conceição de Souza Ailton Cesar Lemes Maria Alice Zarur Coelho |
| author_sort | Aparecida Selsiane Sousa Carvalho |
| collection | DOAJ |
| description | The production of polyunsaturated fatty acids from fish oil, which is related to various health benefits including effects against cardiovascular diseases, antihypertensive, anticancer, antioxidant, antidepression, anti-aging, and anti-arthritis effects, among others, can be advantageously performed through the application of lipase. However, the high cost associated with enzyme production can make the process unfeasible and thus alternative substrates should be investigated to solve these problems. This research aimed to produce lipase by <i>Yarrowia lipolytica</i> IMUFRJ50682 in solid-state fermentation using by-products of the food processing industry (andiroba oil cake and soybean meal) and verify the potential application in the initial hydrolysis of fish oil to further produce polyunsaturated fatty acids in a suitable process. A screening was carried out for the analysis of andiroba oil cake and soybean meal combinations in different proportions (0:100 to 100:0, respectively) at 48 h of the fermentation process. Afterward, the solid matrix composed by soybean meal and andiroba oil cake was supplemented with soy oil and Tween 80 to improve the lipase activity. The enzymatic extract was characterized in relation to the protein profile by electrophoresis. Finally, the enzymatic extract and the solid biocatalyst produced were applied to evaluate the potential hydrolysis of the fish oil in an initial study. Maximum lipolytic activity (63.7 U·g<sup>−1</sup>) was achieved using andiroba oil cake and soybean meal (50:50) after 24 h of fermentation. Soybean oil 1.5% and Tween 80 (0.001%) in an emulsion provided an increase of 1.5-fold (82.52 U·g<sup>−1</sup>) in the enzyme activity. The electrophoretic analysis demonstrated a band between 37 and 40 kDa that may be related to lipase and a band of 75 kDa referring to the α subunit of the β-conglycinin present in soybean meal. After 48 h, the solid biocatalyst showed a higher degree of hydrolysis (DH) (71.0%) than the enzymatic extract (61.5%). The solid biocatalyst was stable during storage at room temperature for 7 months. The production of lipases using Amazon fruit by-product and soybean meal in solid-state fermentation is viable as well as the application of the extract and solid biocatalyst in the initial application for the hydrolysis of fish oil to further produce polyunsaturated fatty acids in an industrially suited process. |
| first_indexed | 2024-03-11T09:02:01Z |
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| spelling | doaj.art-74eb901f87f24dbfbec19f2ae6f51dbb2023-11-16T19:41:02ZengMDPI AGCatalysts2073-43442023-01-0113228910.3390/catal13020289Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as SubstrateAparecida Selsiane Sousa Carvalho0Júlio Cesar Soares Sales1Felipe Valle do Nascimento2Bernardo Dias Ribeiro3Carlos Eduardo Conceição de Souza4Ailton Cesar Lemes5Maria Alice Zarur Coelho6Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilDepartamento de Tecnologia de Processos Bioquímicos, Instituto de Química, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524 Pavilhão Haroldo Lisboa da Cunha, Rio de Janeiro 20550-013, BrazilDepartamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilDepartamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilDepartamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos 149, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilThe production of polyunsaturated fatty acids from fish oil, which is related to various health benefits including effects against cardiovascular diseases, antihypertensive, anticancer, antioxidant, antidepression, anti-aging, and anti-arthritis effects, among others, can be advantageously performed through the application of lipase. However, the high cost associated with enzyme production can make the process unfeasible and thus alternative substrates should be investigated to solve these problems. This research aimed to produce lipase by <i>Yarrowia lipolytica</i> IMUFRJ50682 in solid-state fermentation using by-products of the food processing industry (andiroba oil cake and soybean meal) and verify the potential application in the initial hydrolysis of fish oil to further produce polyunsaturated fatty acids in a suitable process. A screening was carried out for the analysis of andiroba oil cake and soybean meal combinations in different proportions (0:100 to 100:0, respectively) at 48 h of the fermentation process. Afterward, the solid matrix composed by soybean meal and andiroba oil cake was supplemented with soy oil and Tween 80 to improve the lipase activity. The enzymatic extract was characterized in relation to the protein profile by electrophoresis. Finally, the enzymatic extract and the solid biocatalyst produced were applied to evaluate the potential hydrolysis of the fish oil in an initial study. Maximum lipolytic activity (63.7 U·g<sup>−1</sup>) was achieved using andiroba oil cake and soybean meal (50:50) after 24 h of fermentation. Soybean oil 1.5% and Tween 80 (0.001%) in an emulsion provided an increase of 1.5-fold (82.52 U·g<sup>−1</sup>) in the enzyme activity. The electrophoretic analysis demonstrated a band between 37 and 40 kDa that may be related to lipase and a band of 75 kDa referring to the α subunit of the β-conglycinin present in soybean meal. After 48 h, the solid biocatalyst showed a higher degree of hydrolysis (DH) (71.0%) than the enzymatic extract (61.5%). The solid biocatalyst was stable during storage at room temperature for 7 months. The production of lipases using Amazon fruit by-product and soybean meal in solid-state fermentation is viable as well as the application of the extract and solid biocatalyst in the initial application for the hydrolysis of fish oil to further produce polyunsaturated fatty acids in an industrially suited process.https://www.mdpi.com/2073-4344/13/2/289<i>Carapa guianensis</i> Aubletlipase productionsolid-state fermentationsustainability<i>Yarrowia lipolytica</i> |
| spellingShingle | Aparecida Selsiane Sousa Carvalho Júlio Cesar Soares Sales Felipe Valle do Nascimento Bernardo Dias Ribeiro Carlos Eduardo Conceição de Souza Ailton Cesar Lemes Maria Alice Zarur Coelho Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate Catalysts <i>Carapa guianensis</i> Aublet lipase production solid-state fermentation sustainability <i>Yarrowia lipolytica</i> |
| title | Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate |
| title_full | Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate |
| title_fullStr | Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate |
| title_full_unstemmed | Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate |
| title_short | Lipase Production by <i>Yarrowia lipolytica</i> in Solid-State Fermentation Using Amazon Fruit By-Products and Soybean Meal as Substrate |
| title_sort | lipase production by i yarrowia lipolytica i in solid state fermentation using amazon fruit by products and soybean meal as substrate |
| topic | <i>Carapa guianensis</i> Aublet lipase production solid-state fermentation sustainability <i>Yarrowia lipolytica</i> |
| url | https://www.mdpi.com/2073-4344/13/2/289 |
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