Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures

Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures

We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. 2.3.1.85) activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified F...

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Bibliographic Details
Main Authors: Andrea A.F.B.V. José, Lúcia. E. Alvares, Eduardo F. Delgado, Luiz L. Coutinho, Dante P.D. Lanna
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2006-01-01
Series:Genetics and Molecular Biology
Subjects:
mRNA quantification
enzyme activity
swine
cultures of adipose tissue explants
competitive RT-PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100032

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100032

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