Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage
<b>Background/objectives:</b> Aging leads to increased oxidative stress and chronic inflammation in the skin, which contribute to various disorders such as dermatitis and cancer. This study explores the cytoprotective effects of oleanolic acid (OA), a natural triterpenoid compound known...
| প্রধান লেখক: | , , , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
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MDPI AG
2025-02-01
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| মালা: | Pharmaceuticals |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://www.mdpi.com/1424-8247/18/2/238 |
| _version_ | 1826581693078700032 |
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| author | Marzia Vasarri Maria Camilla Bergonzi Manuela Leri Rebecca Castellacci Monica Bucciantini Lucia De Marchi Donatella Degl’Innocenti |
| author_facet | Marzia Vasarri Maria Camilla Bergonzi Manuela Leri Rebecca Castellacci Monica Bucciantini Lucia De Marchi Donatella Degl’Innocenti |
| author_sort | Marzia Vasarri |
| collection | DOAJ |
| description | <b>Background/objectives:</b> Aging leads to increased oxidative stress and chronic inflammation in the skin, which contribute to various disorders such as dermatitis and cancer. This study explores the cytoprotective effects of oleanolic acid (OA), a natural triterpenoid compound known for its potential in mitigating oxidative damage, on human keratinocyte (HaCaT) cells exposed to oxidative stress from tert-butyl hydroperoxide (tBHP). <b>Methods:</b> Using in vitro experiments, we assessed cell viability, reactive oxygen species (ROS) levels, nitric oxide (NO) production, and protein expression following OA pre-treatment. Advanced imaging techniques were employed to visualize protein localization. <b>Results:</b> Results demonstrated that OA significantly improved cell viability and reduced intracellular ROS levels compared with those in controls. Additionally, OA inhibited inducible nitric oxide synthase (iNOS) expression and subsequent nitric oxide release, indicating a modulation of inflammatory responses. Notably, while tBHP activated the Nrf2/HO-1 signaling pathway, OA did not enhance this response, suggesting that OA exerts cytoprotective effects through mechanisms independent of Nrf2 activation. <b>Conclusion:</b> OA shows promise in protecting HaCaT cells from tBHP-induced oxidative stress, highlighting its potential role in promoting skin health and addressing aging-related damage. The study proposes that OA operates through pathways distinct from Nrf2 and MAPKs, paving the way for new therapeutic strategies aimed at improving skin health against oxidative stress. |
| first_indexed | 2025-03-14T14:54:07Z |
| format | Article |
| id | doaj.art-751a2379d42645c2ad80dd5a53692261 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2025-03-14T14:54:07Z |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-751a2379d42645c2ad80dd5a536922612025-02-25T13:42:45ZengMDPI AGPharmaceuticals1424-82472025-02-0118223810.3390/ph18020238Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous DamageMarzia Vasarri0Maria Camilla Bergonzi1Manuela Leri2Rebecca Castellacci3Monica Bucciantini4Lucia De Marchi5Donatella Degl’Innocenti6Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Chemistry “Ugo Schiff”, University of Florence, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyDepartment of Chemistry “Ugo Schiff”, University of Florence, Via Ugo Schiff 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, ItalyVeterinary Teaching Hospital, Department of Veterinary Sciences, University of Pisa, 56124 Pisa, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy<b>Background/objectives:</b> Aging leads to increased oxidative stress and chronic inflammation in the skin, which contribute to various disorders such as dermatitis and cancer. This study explores the cytoprotective effects of oleanolic acid (OA), a natural triterpenoid compound known for its potential in mitigating oxidative damage, on human keratinocyte (HaCaT) cells exposed to oxidative stress from tert-butyl hydroperoxide (tBHP). <b>Methods:</b> Using in vitro experiments, we assessed cell viability, reactive oxygen species (ROS) levels, nitric oxide (NO) production, and protein expression following OA pre-treatment. Advanced imaging techniques were employed to visualize protein localization. <b>Results:</b> Results demonstrated that OA significantly improved cell viability and reduced intracellular ROS levels compared with those in controls. Additionally, OA inhibited inducible nitric oxide synthase (iNOS) expression and subsequent nitric oxide release, indicating a modulation of inflammatory responses. Notably, while tBHP activated the Nrf2/HO-1 signaling pathway, OA did not enhance this response, suggesting that OA exerts cytoprotective effects through mechanisms independent of Nrf2 activation. <b>Conclusion:</b> OA shows promise in protecting HaCaT cells from tBHP-induced oxidative stress, highlighting its potential role in promoting skin health and addressing aging-related damage. The study proposes that OA operates through pathways distinct from Nrf2 and MAPKs, paving the way for new therapeutic strategies aimed at improving skin health against oxidative stress.https://www.mdpi.com/1424-8247/18/2/238oleanolic acidHaCaT cellsoxidative stresstert-butyl-hydroperoxide |
| spellingShingle | Marzia Vasarri Maria Camilla Bergonzi Manuela Leri Rebecca Castellacci Monica Bucciantini Lucia De Marchi Donatella Degl’Innocenti Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage Pharmaceuticals oleanolic acid HaCaT cells oxidative stress tert-butyl-hydroperoxide |
| title | Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage |
| title_full | Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage |
| title_fullStr | Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage |
| title_full_unstemmed | Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage |
| title_short | Protective Effects of Oleanolic Acid on Human Keratinocytes: A Defense Against Exogenous Damage |
| title_sort | protective effects of oleanolic acid on human keratinocytes a defense against exogenous damage |
| topic | oleanolic acid HaCaT cells oxidative stress tert-butyl-hydroperoxide |
| url | https://www.mdpi.com/1424-8247/18/2/238 |
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