Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects
Abstract Background The association of HTRA1 rs11200638 and ARMS2 rs10490924 gene polymorphisms with response to intravitreal ranibizumab therapy among neovascular AMD (nAMD) subjects in Malaysia was determined in this study, followed by the expression of HTRA1 and ARMS2 genes. Results Both single n...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Human Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s40246-019-0197-3 |
| _version_ | 1811300125435232256 |
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| author | Nur Afiqah Mohamad Vasudevan Ramachandran Hazlita Mohd Isa Yoke Mun Chan Nor Fariza Ngah Siew Mooi Ching Fan Kee Hoo Wan Aliaa Wan Sulaiman Liyana Najwa Inche Mat Mohd Hazmi Mohamed |
| author_facet | Nur Afiqah Mohamad Vasudevan Ramachandran Hazlita Mohd Isa Yoke Mun Chan Nor Fariza Ngah Siew Mooi Ching Fan Kee Hoo Wan Aliaa Wan Sulaiman Liyana Najwa Inche Mat Mohd Hazmi Mohamed |
| author_sort | Nur Afiqah Mohamad |
| collection | DOAJ |
| description | Abstract Background The association of HTRA1 rs11200638 and ARMS2 rs10490924 gene polymorphisms with response to intravitreal ranibizumab therapy among neovascular AMD (nAMD) subjects in Malaysia was determined in this study, followed by the expression of HTRA1 and ARMS2 genes. Results Both single nucleotide polymorphisms (SNPs) recorded a significant association between nAMD and controls with HTRA1 rs11200638 at P = 0.018 (OR = 1.52, 95% CI = 1.07–215) and ARMS2 rs10490924 at P < 0.001 (OR = 2.44, 95% CI = 1.75–3.42). An association was also observed in response to ranibizumab for both SNPs in a logistic regression analysis (P < 0.001). The mRNA levels in the HTRA1 variant between responder and non-responder groups were significantly different for the homozygous non-risk GG genotype (P = 0.032). Conclusions The HTRA1 rs11200638 and ARMS2 rs10490924 gene polymorphisms are associated with nAMD among Malaysians. Both gene polymorphisms were also correlated with response to intravitreal ranibizumab therapy based on visual and anatomical outcomes especially the HTRA1 rs11200638 variant. |
| first_indexed | 2024-04-13T06:46:39Z |
| format | Article |
| id | doaj.art-7526802e10414643b2b0fb706a97dae7 |
| institution | Directory Open Access Journal |
| issn | 1479-7364 |
| language | English |
| last_indexed | 2024-04-13T06:46:39Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Human Genomics |
| spelling | doaj.art-7526802e10414643b2b0fb706a97dae72022-12-22T02:57:33ZengBMCHuman Genomics1479-73642019-02-0113111210.1186/s40246-019-0197-3Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjectsNur Afiqah Mohamad0Vasudevan Ramachandran1Hazlita Mohd Isa2Yoke Mun Chan3Nor Fariza Ngah4Siew Mooi Ching5Fan Kee Hoo6Wan Aliaa Wan Sulaiman7Liyana Najwa Inche Mat8Mohd Hazmi Mohamed9Malaysian Research Institute on Ageing, Universiti Putra MalaysiaMalaysian Research Institute on Ageing, Universiti Putra MalaysiaDepartment of Ophthalmology, Universiti Kebangsaan Malaysia Medical CentreMalaysian Research Institute on Ageing, Universiti Putra MalaysiaDepartment of Ophthalmology, Hospital SelayangDepartment of Family Medicine, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Surgery, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaAbstract Background The association of HTRA1 rs11200638 and ARMS2 rs10490924 gene polymorphisms with response to intravitreal ranibizumab therapy among neovascular AMD (nAMD) subjects in Malaysia was determined in this study, followed by the expression of HTRA1 and ARMS2 genes. Results Both single nucleotide polymorphisms (SNPs) recorded a significant association between nAMD and controls with HTRA1 rs11200638 at P = 0.018 (OR = 1.52, 95% CI = 1.07–215) and ARMS2 rs10490924 at P < 0.001 (OR = 2.44, 95% CI = 1.75–3.42). An association was also observed in response to ranibizumab for both SNPs in a logistic regression analysis (P < 0.001). The mRNA levels in the HTRA1 variant between responder and non-responder groups were significantly different for the homozygous non-risk GG genotype (P = 0.032). Conclusions The HTRA1 rs11200638 and ARMS2 rs10490924 gene polymorphisms are associated with nAMD among Malaysians. Both gene polymorphisms were also correlated with response to intravitreal ranibizumab therapy based on visual and anatomical outcomes especially the HTRA1 rs11200638 variant.http://link.springer.com/article/10.1186/s40246-019-0197-3Age-related macular degenerationAge-related maculopathy susceptibility 2High temperature requirement a serine peptidase 1PolymorphismRanibizumab |
| spellingShingle | Nur Afiqah Mohamad Vasudevan Ramachandran Hazlita Mohd Isa Yoke Mun Chan Nor Fariza Ngah Siew Mooi Ching Fan Kee Hoo Wan Aliaa Wan Sulaiman Liyana Najwa Inche Mat Mohd Hazmi Mohamed Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects Human Genomics Age-related macular degeneration Age-related maculopathy susceptibility 2 High temperature requirement a serine peptidase 1 Polymorphism Ranibizumab |
| title | Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects |
| title_full | Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects |
| title_fullStr | Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects |
| title_full_unstemmed | Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects |
| title_short | Association of HTRA1 and ARMS2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age-related macular degenerative subjects |
| title_sort | association of htra1 and arms2 gene polymorphisms with response to intravitreal ranibizumab among neovascular age related macular degenerative subjects |
| topic | Age-related macular degeneration Age-related maculopathy susceptibility 2 High temperature requirement a serine peptidase 1 Polymorphism Ranibizumab |
| url | http://link.springer.com/article/10.1186/s40246-019-0197-3 |
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