Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development
Although decades of studies have produced a generalized model for tetrapod limb development, urodeles deviate from anurans and amniotes in at least two key respects: their limbs exhibit preaxial skeletal differentiation and do not develop an apical ectodermal ridge (AER). Here, we investigated how S...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/48507 |
| _version_ | 1811180613144674304 |
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| author | Sruthi Purushothaman Ahmed Elewa Ashley W Seifert |
| author_facet | Sruthi Purushothaman Ahmed Elewa Ashley W Seifert |
| author_sort | Sruthi Purushothaman |
| collection | DOAJ |
| description | Although decades of studies have produced a generalized model for tetrapod limb development, urodeles deviate from anurans and amniotes in at least two key respects: their limbs exhibit preaxial skeletal differentiation and do not develop an apical ectodermal ridge (AER). Here, we investigated how Sonic hedgehog (Shh) and Fibroblast growth factor (Fgf) signaling regulate limb development in the axolotl. We found that Shh-expressing cells contributed to the most posterior digit, and that inhibiting Shh-signaling inhibited Fgf8 expression, anteroposterior patterning, and distal cell proliferation. In addition to lack of a morphological AER, we found that salamander limbs also lack a molecular AER. We found that amniote and anuran AER-specific Fgfs and their cognate receptors were expressed entirely in the mesenchyme. Broad inhibition of Fgf-signaling demonstrated that this pathway regulates cell proliferation across all three limb axes, in contrast to anurans and amniotes where Fgf-signaling regulates cell survival and proximodistal patterning. |
| first_indexed | 2024-04-11T09:06:13Z |
| format | Article |
| id | doaj.art-752759c9c24e4d2b8ae2e80e01dfdeb1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:06:13Z |
| publishDate | 2019-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-752759c9c24e4d2b8ae2e80e01dfdeb12022-12-22T04:32:37ZengeLife Sciences Publications LtdeLife2050-084X2019-09-01810.7554/eLife.48507Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb developmentSruthi Purushothaman0https://orcid.org/0000-0002-3974-4731Ahmed Elewa1https://orcid.org/0000-0002-1988-7970Ashley W Seifert2https://orcid.org/0000-0001-6576-3664Department of Biology, University of Kentucky, Lexington, United StatesCold Spring Harbor Laboratory, Cold Spring Harbor, United StatesDepartment of Biology, University of Kentucky, Lexington, United StatesAlthough decades of studies have produced a generalized model for tetrapod limb development, urodeles deviate from anurans and amniotes in at least two key respects: their limbs exhibit preaxial skeletal differentiation and do not develop an apical ectodermal ridge (AER). Here, we investigated how Sonic hedgehog (Shh) and Fibroblast growth factor (Fgf) signaling regulate limb development in the axolotl. We found that Shh-expressing cells contributed to the most posterior digit, and that inhibiting Shh-signaling inhibited Fgf8 expression, anteroposterior patterning, and distal cell proliferation. In addition to lack of a morphological AER, we found that salamander limbs also lack a molecular AER. We found that amniote and anuran AER-specific Fgfs and their cognate receptors were expressed entirely in the mesenchyme. Broad inhibition of Fgf-signaling demonstrated that this pathway regulates cell proliferation across all three limb axes, in contrast to anurans and amniotes where Fgf-signaling regulates cell survival and proximodistal patterning.https://elifesciences.org/articles/48507limb developmentSonic hedgehogfibroblast growth factorsalamandermorphogenesis |
| spellingShingle | Sruthi Purushothaman Ahmed Elewa Ashley W Seifert Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development eLife limb development Sonic hedgehog fibroblast growth factor salamander morphogenesis |
| title | Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development |
| title_full | Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development |
| title_fullStr | Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development |
| title_full_unstemmed | Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development |
| title_short | Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development |
| title_sort | fgf signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development |
| topic | limb development Sonic hedgehog fibroblast growth factor salamander morphogenesis |
| url | https://elifesciences.org/articles/48507 |
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