Efficient targeted integration directed by short homology in zebrafish and mammalian cells
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses sh...
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2020-05-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/53968 |