Efficient targeted integration directed by short homology in zebrafish and mammalian cells
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses sh...
Main Authors: | Wesley A Wierson, Jordan M Welker, Maira P Almeida, Carla M Mann, Dennis A Webster, Melanie E Torrie, Trevor J Weiss, Sekhar Kambakam, Macy K Vollbrecht, Merrina Lan, Kenna C McKeighan, Jacklyn Levey, Zhitao Ming, Alec Wehmeier, Christopher S Mikelson, Jeffrey A Haltom, Kristen M Kwan, Chi-Bin Chien, Darius Balciunas, Stephen C Ekker, Karl J Clark, Beau R Webber, Branden S Moriarity, Stacy L Solin, Daniel F Carlson, Drena L Dobbs, Maura McGrail, Jeffrey Essner |
---|---|
Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2020-05-01
|
Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/53968 |
Similar Items
-
Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration
by: Fang Liu, et al.
Published: (2022-06-01) -
Building a Canadian Data Platform under the Strategy for Patient-Oriented Research
by: Kimberlyn McGrail
Published: (2019-11-01) -
Exploring inconsistencies in genome-wide protein function annotations: a machine learning approach
by: Dobbs Drena, et al.
Published: (2007-08-01) -
HomPPI: a class of sequence homology based protein-protein interface prediction methods
by: Dobbs Drena, et al.
Published: (2011-06-01) -
Firefighting operations in high-rise and standpipe-equipped buildings /
by: 503653 McGrail, David M.
Published: (c200)