A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced mice

A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced mice

Summary: Highly enriched germinal center (GC) B cell populations are essential for studying humoral immunity. Current MACS protocols that isolate untouched GC B cells require GC induction and typically require further FACS purification with direct antibody labeling to achieve sufficiently high purit...

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Bibliographic Details
Main Authors: Shreya A. Desikan, Sailee Chavan, Peiqi Ou, Christopher A.J. Roman, Chongmin Huan
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Immunology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002684

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http://www.sciencedirect.com/science/article/pii/S2666166722002684

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