Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function
Normal cardiac automaticity is dependent on the pacemaker cells of the sinoatrial node (SAN). Insufficient cardiac pacemaking leads to the development of sick sinus syndrome (SSS). Since currently available pharmaceutical drugs and implantable pacemakers are only partially effective in managing SSS,...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Journal of Cardiovascular Development and Disease |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2308-3425/9/11/402 |
| _version_ | 1797464969738977280 |
|---|---|
| author | Wei Fan Chao Yang Xiaojie Hou Juyi Wan Bin Liao |
| author_facet | Wei Fan Chao Yang Xiaojie Hou Juyi Wan Bin Liao |
| author_sort | Wei Fan |
| collection | DOAJ |
| description | Normal cardiac automaticity is dependent on the pacemaker cells of the sinoatrial node (SAN). Insufficient cardiac pacemaking leads to the development of sick sinus syndrome (SSS). Since currently available pharmaceutical drugs and implantable pacemakers are only partially effective in managing SSS, there is a critical need for developing targeted mechanism-based therapies to treat SSS. SAN-like pacemaker cells (SANLPCs) are difficult to regenerate in vivo or in vitro because the genes and signaling pathways that regulate SAN development and function have not been fully elucidated. The development of more effective treatments for SSS, including biological pacemakers, requires further understanding of these genes and signaling pathways. Compared with genetic models and bulk RNA sequencing, single-cell RNA sequencing (scRNA-seq) technology promises to advance our understanding of cellular phenotype heterogeneity and molecular regulation during SAN development. This review outlines the key transcriptional networks that control the structure, development, and function of the SAN, with particular attention to SAN markers and signaling pathways detected via scRNA-seq. This review offers insights into the process and transcriptional network of SAN morphogenesis at a single-cell level and discusses current challenges and potential future directions for generating SANLPCs for biological pacemakers. |
| first_indexed | 2024-03-09T18:15:45Z |
| format | Article |
| id | doaj.art-7542fe27b62d47bb977e8c914480aaef |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-03-09T18:15:45Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-7542fe27b62d47bb977e8c914480aaef2023-11-24T08:46:55ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252022-11-0191140210.3390/jcdd9110402Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological FunctionWei Fan0Chao Yang1Xiaojie Hou2Juyi Wan3Bin Liao4Department of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, ChinaDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, ChinaDepartment of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100069, ChinaDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, ChinaDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, ChinaNormal cardiac automaticity is dependent on the pacemaker cells of the sinoatrial node (SAN). Insufficient cardiac pacemaking leads to the development of sick sinus syndrome (SSS). Since currently available pharmaceutical drugs and implantable pacemakers are only partially effective in managing SSS, there is a critical need for developing targeted mechanism-based therapies to treat SSS. SAN-like pacemaker cells (SANLPCs) are difficult to regenerate in vivo or in vitro because the genes and signaling pathways that regulate SAN development and function have not been fully elucidated. The development of more effective treatments for SSS, including biological pacemakers, requires further understanding of these genes and signaling pathways. Compared with genetic models and bulk RNA sequencing, single-cell RNA sequencing (scRNA-seq) technology promises to advance our understanding of cellular phenotype heterogeneity and molecular regulation during SAN development. This review outlines the key transcriptional networks that control the structure, development, and function of the SAN, with particular attention to SAN markers and signaling pathways detected via scRNA-seq. This review offers insights into the process and transcriptional network of SAN morphogenesis at a single-cell level and discusses current challenges and potential future directions for generating SANLPCs for biological pacemakers.https://www.mdpi.com/2308-3425/9/11/402sinoatrial nodesingle-cell RNA sequencingtranscription factorssignaling pathwaysmolecular regulation |
| spellingShingle | Wei Fan Chao Yang Xiaojie Hou Juyi Wan Bin Liao Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function Journal of Cardiovascular Development and Disease sinoatrial node single-cell RNA sequencing transcription factors signaling pathways molecular regulation |
| title | Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function |
| title_full | Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function |
| title_fullStr | Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function |
| title_full_unstemmed | Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function |
| title_short | Novel Insights into the Sinoatrial Node in Single-Cell RNA Sequencing: From Developmental Biology to Physiological Function |
| title_sort | novel insights into the sinoatrial node in single cell rna sequencing from developmental biology to physiological function |
| topic | sinoatrial node single-cell RNA sequencing transcription factors signaling pathways molecular regulation |
| url | https://www.mdpi.com/2308-3425/9/11/402 |
| work_keys_str_mv | AT weifan novelinsightsintothesinoatrialnodeinsinglecellrnasequencingfromdevelopmentalbiologytophysiologicalfunction AT chaoyang novelinsightsintothesinoatrialnodeinsinglecellrnasequencingfromdevelopmentalbiologytophysiologicalfunction AT xiaojiehou novelinsightsintothesinoatrialnodeinsinglecellrnasequencingfromdevelopmentalbiologytophysiologicalfunction AT juyiwan novelinsightsintothesinoatrialnodeinsinglecellrnasequencingfromdevelopmentalbiologytophysiologicalfunction AT binliao novelinsightsintothesinoatrialnodeinsinglecellrnasequencingfromdevelopmentalbiologytophysiologicalfunction |