Characterization of aspartokinase double mutants using a combination of experiments and simulations

Characterization of aspartokinase double mutants using a combination of experiments and simulations

Aspartokinase (AK) is synergistically suppressed by Thr and Lys in the Corynebacterium metabolic pathway. Site-directed mutations can significantly improve AK inhibition. Our previous studies confirmed that sites 379 and 380 were important sites affecting enzyme activity, so we further screen the do...

Full description

Bibliographic Details
Main Authors: Zhijie Chen, Yu Fu, Shimeng Liu, Xinyu Huang, Xiaoting Kong, Zhaojie Mao, Ning Hu, Fengxiang Zhang, Caijing Han
Format: Article
Language:English
Published: Elsevier 2023-02-01
Series:Heliyon
Subjects:
Aspartokinase
Double mutant
Site-directed mutation
MST
MD
Mechanism
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023003407

Internet

http://www.sciencedirect.com/science/article/pii/S2405844023003407

Similar Items