Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways
Aspernolide A, a butyrolactone secondary metabolite, was purified from the endophytic fungus Cladosporium cladosporioides derived from roots of Camptotheca acuminata Decne. In this study, the antitumor activity and mechanisms of aspernolide A on human laryngeal cancer Hep-2 and TU212 cells were stud...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/24/6/1074 |
| _version_ | 1819200045235306496 |
|---|---|
| author | Chang Liu Hong Liu Yanzhang Wen Huiqi Huang Ji Hao Yibing Lv Rui Qin Xinzhou Yang |
| author_facet | Chang Liu Hong Liu Yanzhang Wen Huiqi Huang Ji Hao Yibing Lv Rui Qin Xinzhou Yang |
| author_sort | Chang Liu |
| collection | DOAJ |
| description | Aspernolide A, a butyrolactone secondary metabolite, was purified from the endophytic fungus Cladosporium cladosporioides derived from roots of Camptotheca acuminata Decne. In this study, the antitumor activity and mechanisms of aspernolide A on human laryngeal cancer Hep-2 and TU212 cells were studied by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, morphological observation and Western blotting. The results showed that aspernolide A significantly inhibited the proliferation of Hep-2 and TU212 cells in dose- and time-dependent manners. Morphological changes of apoptotic cells could be observed under an inverted microscope, such as irregular margins, decreased adherence ability and chromatin condensation. The expressions of Bax, Caspase-9, Caspase-3 and PARP (poly ADP-ribose polymerase) increased with the increase of dosage while Bcl-2 decreased, suggesting that the apoptotic mechanism might be related to the mitochondrial apoptotic pathway. Moreover, the expression of the phosphorylation of STAT3 decreased with the increase of dosage, suggesting that the apoptotic mechanism might be related to the STAT3 signaling pathway. All these conclusions indicated that aspernolide A has the potential anti-laryngocarcinoma effects. |
| first_indexed | 2024-12-23T03:25:59Z |
| format | Article |
| id | doaj.art-758f40e5d09445ca8c041e8fea002ac9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T03:25:59Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-758f40e5d09445ca8c041e8fea002ac92022-12-21T18:01:50ZengMDPI AGMolecules1420-30492019-03-01246107410.3390/molecules24061074molecules24061074Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling PathwaysChang Liu0Hong Liu1Yanzhang Wen2Huiqi Huang3Ji Hao4Yibing Lv5Rui Qin6Xinzhou Yang7College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaCollege of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaCollege of Life Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, ChinaAspernolide A, a butyrolactone secondary metabolite, was purified from the endophytic fungus Cladosporium cladosporioides derived from roots of Camptotheca acuminata Decne. In this study, the antitumor activity and mechanisms of aspernolide A on human laryngeal cancer Hep-2 and TU212 cells were studied by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, morphological observation and Western blotting. The results showed that aspernolide A significantly inhibited the proliferation of Hep-2 and TU212 cells in dose- and time-dependent manners. Morphological changes of apoptotic cells could be observed under an inverted microscope, such as irregular margins, decreased adherence ability and chromatin condensation. The expressions of Bax, Caspase-9, Caspase-3 and PARP (poly ADP-ribose polymerase) increased with the increase of dosage while Bcl-2 decreased, suggesting that the apoptotic mechanism might be related to the mitochondrial apoptotic pathway. Moreover, the expression of the phosphorylation of STAT3 decreased with the increase of dosage, suggesting that the apoptotic mechanism might be related to the STAT3 signaling pathway. All these conclusions indicated that aspernolide A has the potential anti-laryngocarcinoma effects.http://www.mdpi.com/1420-3049/24/6/1074aspernolide Acamptotheca acuminata decnecladosporium cladosporioidesanticancerlaryngeal cancerapoptosismitochondrial pathwaySTAT3 |
| spellingShingle | Chang Liu Hong Liu Yanzhang Wen Huiqi Huang Ji Hao Yibing Lv Rui Qin Xinzhou Yang Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways Molecules aspernolide A camptotheca acuminata decne cladosporium cladosporioides anticancer laryngeal cancer apoptosis mitochondrial pathway STAT3 |
| title | Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways |
| title_full | Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways |
| title_fullStr | Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways |
| title_full_unstemmed | Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways |
| title_short | Aspernolide A Inhibits the Proliferation of Human Laryngeal Carcinoma Cells through the Mitochondrial Apoptotic and STAT3 Signaling Pathways |
| title_sort | aspernolide a inhibits the proliferation of human laryngeal carcinoma cells through the mitochondrial apoptotic and stat3 signaling pathways |
| topic | aspernolide A camptotheca acuminata decne cladosporium cladosporioides anticancer laryngeal cancer apoptosis mitochondrial pathway STAT3 |
| url | http://www.mdpi.com/1420-3049/24/6/1074 |
| work_keys_str_mv | AT changliu aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT hongliu aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT yanzhangwen aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT huiqihuang aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT jihao aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT yibinglv aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT ruiqin aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways AT xinzhouyang aspernolideainhibitstheproliferationofhumanlaryngealcarcinomacellsthroughthemitochondrialapoptoticandstat3signalingpathways |