Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response
Abstract Bacillus sp. BSp.3/aM, a beneficial rhizobacteria, was analyzed for the ability to improve plant health of chili by suppressing anthracnose disease. In the dual culture assay, the bacterium Bacillus sp. BSp.3/aM was found inhibitory to Colletotrichum capsica (6 mm). Further, upon seed primi...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-07-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41938-019-0148-2 |
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| author | Naveen Jayapala Navya Hulikunte Mallikarjunaiah Hariprasad Puttaswamy Hithamani Gavirangappa Niranjana Siddapura Ramachandrappa |
| author_facet | Naveen Jayapala Navya Hulikunte Mallikarjunaiah Hariprasad Puttaswamy Hithamani Gavirangappa Niranjana Siddapura Ramachandrappa |
| author_sort | Naveen Jayapala |
| collection | DOAJ |
| description | Abstract Bacillus sp. BSp.3/aM, a beneficial rhizobacteria, was analyzed for the ability to improve plant health of chili by suppressing anthracnose disease. In the dual culture assay, the bacterium Bacillus sp. BSp.3/aM was found inhibitory to Colletotrichum capsica (6 mm). Further, upon seed priming, it reduced the seed-borne incidence of C. capsici (2%) and improved seedling vigor (1374 ± 7.15 vigor index) and germination (98 ± 0.57 %) of chili seedlings. Under greenhouse conditions, seed priming resulted in reducing the anthracnose disease incidence up to 20%. Induction of resistance against invading pathogen is through enhancing the activities of defense-related enzymes and higher accumulation of phenolic compounds in the host plant. The activity of phenylalanine ammonia-lyase (PAL; 95 units) was more at 48 hpi; peroxidase (POX; 6.49 units) at 24 hpi; polyphenol oxidase (PPO; 5.81 units) at 24 hpi and lipoxygenase (LOX; 9.9units) at 24 hpi. Maximum accumulation of the phenolics and chitinase accumulation was observed in BSp.3/aM + pathogen treated seedlings 120 hpi (94.7 μg/g tissue) and at 96 hpi (9.36 units), respectively. Thus, increased activities of defense-related enzymes (PAL, POX, PPO, LOX, and chitinase) correlated well with the decreased anthracnose incidence. Induced systemic resistance (ISR) mediated by PGPR was due to the upregulation of defense-related enzymes and by the accumulation of phenolic compounds. |
| first_indexed | 2024-12-13T02:46:50Z |
| format | Article |
| id | doaj.art-759d6d91457d48b8b19b0fe5247402b5 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-12-13T02:46:50Z |
| publishDate | 2019-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-759d6d91457d48b8b19b0fe5247402b52022-12-22T00:02:10ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422019-07-012911910.1186/s41938-019-0148-2Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense responseNaveen Jayapala0Navya Hulikunte Mallikarjunaiah1Hariprasad Puttaswamy2Hithamani Gavirangappa3Niranjana Siddapura Ramachandrappa4Department of Studies in Biotechnology, University of MysoreDepartment of Studies in Biotechnology, University of MysoreCentre for Rural Development and Technology, Indian Institute of Technology, DelhiCSIR-Central Food Technological Research InstituteDepartment of Studies in Biotechnology, University of MysoreAbstract Bacillus sp. BSp.3/aM, a beneficial rhizobacteria, was analyzed for the ability to improve plant health of chili by suppressing anthracnose disease. In the dual culture assay, the bacterium Bacillus sp. BSp.3/aM was found inhibitory to Colletotrichum capsica (6 mm). Further, upon seed priming, it reduced the seed-borne incidence of C. capsici (2%) and improved seedling vigor (1374 ± 7.15 vigor index) and germination (98 ± 0.57 %) of chili seedlings. Under greenhouse conditions, seed priming resulted in reducing the anthracnose disease incidence up to 20%. Induction of resistance against invading pathogen is through enhancing the activities of defense-related enzymes and higher accumulation of phenolic compounds in the host plant. The activity of phenylalanine ammonia-lyase (PAL; 95 units) was more at 48 hpi; peroxidase (POX; 6.49 units) at 24 hpi; polyphenol oxidase (PPO; 5.81 units) at 24 hpi and lipoxygenase (LOX; 9.9units) at 24 hpi. Maximum accumulation of the phenolics and chitinase accumulation was observed in BSp.3/aM + pathogen treated seedlings 120 hpi (94.7 μg/g tissue) and at 96 hpi (9.36 units), respectively. Thus, increased activities of defense-related enzymes (PAL, POX, PPO, LOX, and chitinase) correlated well with the decreased anthracnose incidence. Induced systemic resistance (ISR) mediated by PGPR was due to the upregulation of defense-related enzymes and by the accumulation of phenolic compounds.http://link.springer.com/article/10.1186/s41938-019-0148-2ChilianthracnoseRhizobacteriaBiocontrolDefense-related enzymes (PALPOX |
| spellingShingle | Naveen Jayapala Navya Hulikunte Mallikarjunaiah Hariprasad Puttaswamy Hithamani Gavirangappa Niranjana Siddapura Ramachandrappa Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response Egyptian Journal of Biological Pest Control Chili anthracnose Rhizobacteria Biocontrol Defense-related enzymes (PAL POX |
| title | Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response |
| title_full | Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response |
| title_fullStr | Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response |
| title_full_unstemmed | Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response |
| title_short | Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response |
| title_sort | rhizobacteria bacillus spp induce resistance against anthracnose disease in chili capsicum annuum l through activating host defense response |
| topic | Chili anthracnose Rhizobacteria Biocontrol Defense-related enzymes (PAL POX |
| url | http://link.springer.com/article/10.1186/s41938-019-0148-2 |
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