Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases

Development of a portable DNA extraction and cross-priming amplification (CPA) tool for rapid in-situ visual diagnosis of plant diseases

Abstract Plant pathogens cause severe losses to crop yields and economic returns in agriculture. Despite plant tissue DNA extraction of typically constituting a preliminary step in nucleic acid-based molecular diagnostics, such lab-based methods can be time-consuming and arduous to complete many sam...

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Bibliographic Details
Main Authors: Jie Li, Juan Du, Shengzhican Li, Jiali Dong, Jiahan Ying, Yuehao Gu, Jie Lu, Xinyu Zeng, Philip Kear, Daolong Dou, Xiaodan Wang
Format: Article
Language:English
Published: BMC 2023-06-01
Series:Phytopathology Research
Subjects:
DNA extraction device
Steel microneedle array
Cross-priming amplification
Phytophthora infestans
Pathogen detection
Sample-to-answer
Online Access:https://doi.org/10.1186/s42483-023-00179-9

Internet

https://doi.org/10.1186/s42483-023-00179-9

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