Immunoprecipitation and Western blot-based detection of protein O-GlcNAcylation in cells

Immunoprecipitation and Western blot-based detection of protein O-GlcNAcylation in cells

Summary: Detection of protein O-GlcNAcylation could be challenging. By using the host-cell factor 1 (HCF-1), a known O-GlcNAcylated protein, we immunoprecipitated HCF-1 from transfected HEK293T cells or endogenous HCF-1 from HeLa cells to detect its O-GlcNAc levels by Western blotting. We also take...

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Bibliographic Details
Main Authors: Oumaima Ahmed, Malik Affar, Louis Masclef, Mohamed Echbicheb, Mila Gushul-Leclaire, Benjamin Estavoyer, David J. Vocadlo, El Bachir Affar
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721008145

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http://www.sciencedirect.com/science/article/pii/S2666166721008145

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