Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR

Evaluation of suitable reference genes for gene expression studies in the developing mouse cortex using RT-qPCR

Abstract Background Real-time quantitative PCR (RT-qPCR) is a widely used method to investigate gene expression in neuroscience studies. Accurate relative quantification of RT-qPCR requires the selection of reference genes that are stably expressed across the experimental conditions and tissues of i...

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Bibliographic Details
Main Authors: Ananya Uppalapati, Timothy Wang, Lena H. Nguyen
Format: Article
Language:English
Published: BMC 2025-02-01
Series:BMC Neuroscience
Subjects:
Cerebral cortex
Neurodevelopment
Mouse
RT-qPCR
Gene expression
Reference genes
Online Access:https://doi.org/10.1186/s12868-025-00934-y

Internet

https://doi.org/10.1186/s12868-025-00934-y

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