Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing
ObjectiveTo study the molecular characteristics and antibiotic resistance of Bacillus cereus (B. cereus) from foods in Shanghai.MethodsThe B. cereus isolates were characterized using antibiotic susceptibility tests and whole genome sequencing (WGS). BioNumerics software was used to analyze and assem...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The Editorial Office of Chinese Journal of Food Hygiene
2021-09-01
|
| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20210502?st=article_issue |
| _version_ | 1797976860052684800 |
|---|---|
| author | ZHANG Hongzhi LIU Xuewei GU Qifang CHANG Zhaoyu ZHU Yanqi ZHANG Xi |
| author_facet | ZHANG Hongzhi LIU Xuewei GU Qifang CHANG Zhaoyu ZHU Yanqi ZHANG Xi |
| author_sort | ZHANG Hongzhi |
| collection | DOAJ |
| description | ObjectiveTo study the molecular characteristics and antibiotic resistance of Bacillus cereus (B. cereus) from foods in Shanghai.MethodsThe B. cereus isolates were characterized using antibiotic susceptibility tests and whole genome sequencing (WGS). BioNumerics software was used to analyze and assemble WGS data. Then, multilocus sequence typing (MLST), virulence genes profiles and antibiotic resistant genes were analyzed using assembled genome sequences. The antimicrobial susceptibility was investigated. Furthermore, STs of B. cereus in this study were compared with those from PubMLST database.ResultsIn this study, all 37 isolates were confirmed as B. cereus isolates. The 37 B. cereus were typed into 34 STs with 7 new STs. The result of STs showed high diversity. All 37 B. cereus isolates had nheA, nheB and nheC genes. The positive rate of hblACD genes was 37.8%. However, only 16.2% of B. cereus had ces genes. Furthermore, two ST26 B. cereus isolates with ces genes from ready-to-eat food were identified. The antibiotic resistance rate to Ampicillin, Penicillin and Oxacillin was high, which was not consistent with antibiotic resistant genes.ConclusionThe STs of 37 B. cereus from food in Shanghai showed high genetic diversity, which suggested the molecular surveillance should be enhanced. Fundamental data can be obtained using whole genome sequencing for tracing B. cereus isolates and analyzing pathogenicity. |
| first_indexed | 2024-04-11T04:57:41Z |
| format | Article |
| id | doaj.art-75c36566871a4ab6b750f5652f445297 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:57:41Z |
| publishDate | 2021-09-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-75c36566871a4ab6b750f5652f4452972022-12-26T07:34:26ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562021-09-01330552953510.13590/j.cjfh.2021.05.0021004-8456(2021)05-0529-07Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencingZHANG Hongzhi0LIU Xuewei1GU Qifang2CHANG Zhaoyu3ZHU Yanqi4ZHANG Xi5Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaObjectiveTo study the molecular characteristics and antibiotic resistance of Bacillus cereus (B. cereus) from foods in Shanghai.MethodsThe B. cereus isolates were characterized using antibiotic susceptibility tests and whole genome sequencing (WGS). BioNumerics software was used to analyze and assemble WGS data. Then, multilocus sequence typing (MLST), virulence genes profiles and antibiotic resistant genes were analyzed using assembled genome sequences. The antimicrobial susceptibility was investigated. Furthermore, STs of B. cereus in this study were compared with those from PubMLST database.ResultsIn this study, all 37 isolates were confirmed as B. cereus isolates. The 37 B. cereus were typed into 34 STs with 7 new STs. The result of STs showed high diversity. All 37 B. cereus isolates had nheA, nheB and nheC genes. The positive rate of hblACD genes was 37.8%. However, only 16.2% of B. cereus had ces genes. Furthermore, two ST26 B. cereus isolates with ces genes from ready-to-eat food were identified. The antibiotic resistance rate to Ampicillin, Penicillin and Oxacillin was high, which was not consistent with antibiotic resistant genes.ConclusionThe STs of 37 B. cereus from food in Shanghai showed high genetic diversity, which suggested the molecular surveillance should be enhanced. Fundamental data can be obtained using whole genome sequencing for tracing B. cereus isolates and analyzing pathogenicity.http://www.zgspws.com/zgspwszz/article/abstract/20210502?st=article_issuebacillus cereuswhole genome sequencingmultilocus sequence typingvirulence genes profilesresistance |
| spellingShingle | ZHANG Hongzhi LIU Xuewei GU Qifang CHANG Zhaoyu ZHU Yanqi ZHANG Xi Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing Zhongguo shipin weisheng zazhi bacillus cereus whole genome sequencing multilocus sequence typing virulence genes profiles resistance |
| title | Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing |
| title_full | Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing |
| title_fullStr | Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing |
| title_full_unstemmed | Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing |
| title_short | Molecular characteristics and antibiotic resistance of Bacillus cereus from foods using whole genome sequencing |
| title_sort | molecular characteristics and antibiotic resistance of bacillus cereus from foods using whole genome sequencing |
| topic | bacillus cereus whole genome sequencing multilocus sequence typing virulence genes profiles resistance |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20210502?st=article_issue |
| work_keys_str_mv | AT zhanghongzhi molecularcharacteristicsandantibioticresistanceofbacilluscereusfromfoodsusingwholegenomesequencing AT liuxuewei molecularcharacteristicsandantibioticresistanceofbacilluscereusfromfoodsusingwholegenomesequencing AT guqifang molecularcharacteristicsandantibioticresistanceofbacilluscereusfromfoodsusingwholegenomesequencing AT changzhaoyu molecularcharacteristicsandantibioticresistanceofbacilluscereusfromfoodsusingwholegenomesequencing AT zhuyanqi molecularcharacteristicsandantibioticresistanceofbacilluscereusfromfoodsusingwholegenomesequencing AT zhangxi molecularcharacteristicsandantibioticresistanceofbacilluscereusfromfoodsusingwholegenomesequencing |