Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis
An automated solid phase extraction (SPE) protocol to determine carbamazepine in human serum has been developed and validated using a simple, rabid and sensitive liquid chromatography-based bio-analytical method. Extraction of carbamazepine was carried out using an on-line SPE tool of a short protei...
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MDPI AG
2023-01-01
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| author | Heba Sonbol Hager Ebrahim Monika Malak Ahmed Ali Yasmine Aboulella Ghada Hadad Samy Emara Ahmed Shawky |
| author_facet | Heba Sonbol Hager Ebrahim Monika Malak Ahmed Ali Yasmine Aboulella Ghada Hadad Samy Emara Ahmed Shawky |
| author_sort | Heba Sonbol |
| collection | DOAJ |
| description | An automated solid phase extraction (SPE) protocol to determine carbamazepine in human serum has been developed and validated using a simple, rabid and sensitive liquid chromatography-based bio-analytical method. Extraction of carbamazepine was carried out using an on-line SPE tool of a short protein-coated (PC) ODS silica pre-column (PC-ODS-pre-column) and phosphate buffer saline (PBS) with a pH of 7.4 as an extraction solvent. There are two distinct chromatographic modes used by PC-ODS-pre-column. While carbamazepine trapping required reversed-phase liquid chromatography, proteins were extracted from serum samples using PBS by size-exclusion liquid chromatography. Then, carbamazepine was eluted from the PC-ODS-pre-column onto the quantification position using a mixture of methanol-distilled deionized water (50:50, <i>v</i>/<i>v</i>) as an eluent and ODS analytical column. At room temperature (22 ± 1 °C), carbamazepine was completely separated from the co-eluted matrix components and detected at 230 nm. Carbamazepine’s linearity was obtained at concentrations ranging from 50 to 10,000 ng/mL. With good accuracy and precision, carbamazepine recoveries in serum samples ranged from 86.14 to 97.82%. The extraction step was conducted using PBS as a safe and green extraction solvent, making this protocol both cost-effective and ecologically safe. |
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| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T08:09:55Z |
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| spelling | doaj.art-75e52b4f5ecf46d9aabc8787c67beaf92023-11-16T23:13:57ZengMDPI AGSeparations2297-87392023-01-011027110.3390/separations10020071Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic AnalysisHeba Sonbol0Hager Ebrahim1Monika Malak2Ahmed Ali3Yasmine Aboulella4Ghada Hadad5Samy Emara6Ahmed Shawky7Pharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptResearch Center, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptResearch Center, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptPharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Ismailia Road, Cairo 44971, EgyptAn automated solid phase extraction (SPE) protocol to determine carbamazepine in human serum has been developed and validated using a simple, rabid and sensitive liquid chromatography-based bio-analytical method. Extraction of carbamazepine was carried out using an on-line SPE tool of a short protein-coated (PC) ODS silica pre-column (PC-ODS-pre-column) and phosphate buffer saline (PBS) with a pH of 7.4 as an extraction solvent. There are two distinct chromatographic modes used by PC-ODS-pre-column. While carbamazepine trapping required reversed-phase liquid chromatography, proteins were extracted from serum samples using PBS by size-exclusion liquid chromatography. Then, carbamazepine was eluted from the PC-ODS-pre-column onto the quantification position using a mixture of methanol-distilled deionized water (50:50, <i>v</i>/<i>v</i>) as an eluent and ODS analytical column. At room temperature (22 ± 1 °C), carbamazepine was completely separated from the co-eluted matrix components and detected at 230 nm. Carbamazepine’s linearity was obtained at concentrations ranging from 50 to 10,000 ng/mL. With good accuracy and precision, carbamazepine recoveries in serum samples ranged from 86.14 to 97.82%. The extraction step was conducted using PBS as a safe and green extraction solvent, making this protocol both cost-effective and ecologically safe.https://www.mdpi.com/2297-8739/10/2/71carbamazepineprotein-coated ODS silica pre-columnhuman serumon-line solid phase extractionUV detection |
| spellingShingle | Heba Sonbol Hager Ebrahim Monika Malak Ahmed Ali Yasmine Aboulella Ghada Hadad Samy Emara Ahmed Shawky Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis Separations carbamazepine protein-coated ODS silica pre-column human serum on-line solid phase extraction UV detection |
| title | Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis |
| title_full | Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis |
| title_fullStr | Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis |
| title_full_unstemmed | Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis |
| title_short | Application of a Small Protein-Coated Column to Trap, Extract and Enrich Carbamazepine Directly from Human Serum for Direct Chromatographic Analysis |
| title_sort | application of a small protein coated column to trap extract and enrich carbamazepine directly from human serum for direct chromatographic analysis |
| topic | carbamazepine protein-coated ODS silica pre-column human serum on-line solid phase extraction UV detection |
| url | https://www.mdpi.com/2297-8739/10/2/71 |
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