Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa

Abstract Background Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. Results In this study, we established a transformation procedure involving chemical-inducible...

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Bibliographic Details
Main Authors: Yuanyuan Liu, Li Zhang, Cheng Li, Yunqiang Yang, Yuanwen Duan, Yongping Yang, Xudong Sun
Format: Article
Language:English
Published: BMC 2022-08-01
Series:Plant Methods
Subjects:
Online Access:https://doi.org/10.1186/s13007-022-00931-w