Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa
Abstract Background Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. Results In this study, we established a transformation procedure involving chemical-inducible...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13007-022-00931-w |
| _version_ | 1828294036449918976 |
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| author | Yuanyuan Liu Li Zhang Cheng Li Yunqiang Yang Yuanwen Duan Yongping Yang Xudong Sun |
| author_facet | Yuanyuan Liu Li Zhang Cheng Li Yunqiang Yang Yuanwen Duan Yongping Yang Xudong Sun |
| author_sort | Yuanyuan Liu |
| collection | DOAJ |
| description | Abstract Background Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. Results In this study, we established a transformation procedure involving chemical-inducible activation of the BrrWUSa gene, which resulted in high transformation frequencies of turnip. Estradiol-inducible BrrWUSa transgenic plants were fertile and showed no obvious developmental defects. Furthermore, we used CRISPR/Cas9 gene-editing technology to edit BrrTCP4b and generated 20 BrrTCP4b-edited seedlings with an increase in leaf trichome number. Conclusion The results demonstrate that BrrWUSa improves the regeneration efficiency in turnip. The transformation procedure represents a promising strategy to improve genetic transformation and for functional characterization of genes in turnip. |
| first_indexed | 2024-04-13T11:30:59Z |
| format | Article |
| id | doaj.art-7611c4ac6bd240ea8337d92f29a68b46 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-04-13T11:30:59Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-7611c4ac6bd240ea8337d92f29a68b462022-12-22T02:48:34ZengBMCPlant Methods1746-48112022-08-011811810.1186/s13007-022-00931-wEstablishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapaYuanyuan Liu0Li Zhang1Cheng Li2Yunqiang Yang3Yuanwen Duan4Yongping Yang5Xudong Sun6Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of SciencesAbstract Background Genome editing is essential for crop molecular breeding. However, gene editing in turnip (Brassica rapa var. rapa) have not been reported owing to the very low transformation efficiency. Results In this study, we established a transformation procedure involving chemical-inducible activation of the BrrWUSa gene, which resulted in high transformation frequencies of turnip. Estradiol-inducible BrrWUSa transgenic plants were fertile and showed no obvious developmental defects. Furthermore, we used CRISPR/Cas9 gene-editing technology to edit BrrTCP4b and generated 20 BrrTCP4b-edited seedlings with an increase in leaf trichome number. Conclusion The results demonstrate that BrrWUSa improves the regeneration efficiency in turnip. The transformation procedure represents a promising strategy to improve genetic transformation and for functional characterization of genes in turnip.https://doi.org/10.1186/s13007-022-00931-wTurnipBrassica rapa var. rapaBrrWUSaGenetic transformationGenome editing |
| spellingShingle | Yuanyuan Liu Li Zhang Cheng Li Yunqiang Yang Yuanwen Duan Yongping Yang Xudong Sun Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa Plant Methods Turnip Brassica rapa var. rapa BrrWUSa Genetic transformation Genome editing |
| title | Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa |
| title_full | Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa |
| title_fullStr | Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa |
| title_full_unstemmed | Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa |
| title_short | Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa |
| title_sort | establishment of agrobacterium mediated genetic transformation and application of crispr cas9 genome editing system to brassica rapa var rapa |
| topic | Turnip Brassica rapa var. rapa BrrWUSa Genetic transformation Genome editing |
| url | https://doi.org/10.1186/s13007-022-00931-w |
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