Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR

Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR detection difficult due to the complex nature of the...

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Bibliographic Details
Main Authors: Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irène Iugovaz, Neda Nasheri
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/10/8/1804