Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio)
Since the aquaculture industry is currently observing a deterioration in the flesh quality of farmed fish, the use of nutrients as additives to improve the flesh quality of farmed fish species is a viable strategy. The aim of this study was to investigate the effect of dietary D-ribose (RI) on the n...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-06-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654522001731 |
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| author | Wanjie Cai Lele Fu Cui Liu Linyue He Haokun Liu Dong Han Xiaoming Zhu Yunxia Yang Junyan Jin Shouqi Xie |
| author_facet | Wanjie Cai Lele Fu Cui Liu Linyue He Haokun Liu Dong Han Xiaoming Zhu Yunxia Yang Junyan Jin Shouqi Xie |
| author_sort | Wanjie Cai |
| collection | DOAJ |
| description | Since the aquaculture industry is currently observing a deterioration in the flesh quality of farmed fish, the use of nutrients as additives to improve the flesh quality of farmed fish species is a viable strategy. The aim of this study was to investigate the effect of dietary D-ribose (RI) on the nutritional value, texture and flavour of gibel carp (Carassius auratus gibelio). Four diets were formulated containing exogenous RI at 4 gradient levels: 0 (Control), 0.15% (0.15RI), 0.30% (0.30RI) and 0.45% (0.45RI). A total of 240 fish (150 ± 0.31 g) were randomly distributed into 12 fibreglass tanks (150 L per tank). Triplicate tanks were randomly assigned to each diet. The feeding trial was carried out in an indoor recirculating aquaculture system for 60 d. After the feeding trial, the muscle and liver of gibel carp were analysed. The results showed that RI supplementation did not result in any negative impact on the growth performance and 0.30RI supplementation significantly increased the whole-body protein content compared to the control group. The contents of collagen and glycogen in muscle were enhanced by RI supplementation. The alterations in the flesh indicated that RI supplementation improved the texture of the flesh in terms of its water-holding capacity and hardness, therefore improving the taste. Dietary RI facilitated the deposition of amino acids and fatty acids in the muscle that contributed to the meaty taste and nutritional value. Furthermore, a combination of metabolomics and expression of key genes in liver and muscle revealed that 0.30RI activated the purine metabolism pathways by supplementing the substrate for nucleotide synthesis and thereby promoting the deposition of flavour substance in flesh. This study offers a new approach for providing healthy, nutritious and flavourful aquatic products. |
| first_indexed | 2024-03-13T05:39:35Z |
| format | Article |
| id | doaj.art-761df0c0fb844ed59bc0364536eae7a1 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-03-13T05:39:35Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-761df0c0fb844ed59bc0364536eae7a12023-06-14T04:33:40ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452023-06-01135063Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio)Wanjie Cai0Lele Fu1Cui Liu2Linyue He3Haokun Liu4Dong Han5Xiaoming Zhu6Yunxia Yang7Junyan Jin8Shouqi Xie9State Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Corresponding author.State Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China; Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed, Wuhan, 430072, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed, Wuhan, 430072, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaState Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China; Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed, Wuhan, 430072, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, 430072 Wuhan, ChinaSince the aquaculture industry is currently observing a deterioration in the flesh quality of farmed fish, the use of nutrients as additives to improve the flesh quality of farmed fish species is a viable strategy. The aim of this study was to investigate the effect of dietary D-ribose (RI) on the nutritional value, texture and flavour of gibel carp (Carassius auratus gibelio). Four diets were formulated containing exogenous RI at 4 gradient levels: 0 (Control), 0.15% (0.15RI), 0.30% (0.30RI) and 0.45% (0.45RI). A total of 240 fish (150 ± 0.31 g) were randomly distributed into 12 fibreglass tanks (150 L per tank). Triplicate tanks were randomly assigned to each diet. The feeding trial was carried out in an indoor recirculating aquaculture system for 60 d. After the feeding trial, the muscle and liver of gibel carp were analysed. The results showed that RI supplementation did not result in any negative impact on the growth performance and 0.30RI supplementation significantly increased the whole-body protein content compared to the control group. The contents of collagen and glycogen in muscle were enhanced by RI supplementation. The alterations in the flesh indicated that RI supplementation improved the texture of the flesh in terms of its water-holding capacity and hardness, therefore improving the taste. Dietary RI facilitated the deposition of amino acids and fatty acids in the muscle that contributed to the meaty taste and nutritional value. Furthermore, a combination of metabolomics and expression of key genes in liver and muscle revealed that 0.30RI activated the purine metabolism pathways by supplementing the substrate for nucleotide synthesis and thereby promoting the deposition of flavour substance in flesh. This study offers a new approach for providing healthy, nutritious and flavourful aquatic products.http://www.sciencedirect.com/science/article/pii/S2405654522001731D-RiboseFlavourFlesh qualityInosine monophosphateMetabolomicPurine metabolism |
| spellingShingle | Wanjie Cai Lele Fu Cui Liu Linyue He Haokun Liu Dong Han Xiaoming Zhu Yunxia Yang Junyan Jin Shouqi Xie Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio) Animal Nutrition D-Ribose Flavour Flesh quality Inosine monophosphate Metabolomic Purine metabolism |
| title | Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio) |
| title_full | Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio) |
| title_fullStr | Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio) |
| title_full_unstemmed | Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio) |
| title_short | Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Carassius auratus gibelio) |
| title_sort | dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp carassius auratus gibelio |
| topic | D-Ribose Flavour Flesh quality Inosine monophosphate Metabolomic Purine metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2405654522001731 |
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