Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L.
The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned an ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly in leaf and flower. AaPYL9 protein can be localized in both nucleus and...
| Egile Nagusiak: | , , , , , , , , |
|---|---|
| Formatua: | Artikulua |
| Hizkuntza: | English |
| Argitaratua: |
Public Library of Science (PLoS)
2013-01-01
|
| Saila: | PLoS ONE |
| Sarrera elektronikoa: | http://europepmc.org/articles/PMC3577733?pdf=render |
| _version_ | 1828875557424594944 |
|---|---|
| author | Fangyuan Zhang Xu Lu Zongyou Lv Ling Zhang Mengmeng Zhu Weiming Jiang Guofeng Wang Xiaofen Sun Kexuan Tang |
| author_facet | Fangyuan Zhang Xu Lu Zongyou Lv Ling Zhang Mengmeng Zhu Weiming Jiang Guofeng Wang Xiaofen Sun Kexuan Tang |
| author_sort | Fangyuan Zhang |
| collection | DOAJ |
| description | The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned an ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly in leaf and flower. AaPYL9 protein can be localized in both nucleus and cytoplasm. Yeast two-hybrid assay shows AaPYL9 can specifically interact with AtABI1 but not with AtABI2, AtHAB1 or AtHAB2. ABA can enhance the interaction between AaPYL9 and AtABI1 while AaPYL9-89 Pro→Ser and AaPYL9-116 His→Ala point mutations abolishes the interaction. BiFC assay shows that AaPYL9 interacts with AtABI1 in nucleus in planta. Transgenic Arabidopsis plants over-expressing AaPYL9 are more sensitive to ABA in the seed germination and primary root growth than wild type. Consistent with this, ABA report genes have higher expression in AaPYL9 overexpressing plants compared to wild type after ABA treatment. Moreover, overexpression of AaPYL9 in A. annua increases not only drought tolerance, but also artemisinin content after ABA treatment, with significant enhancement of the expression of key genes in artemisinin biosynthesis. This study provides a way to develop A. annua with high-yielding artemisinin and high drought resistance. |
| first_indexed | 2024-12-13T08:01:26Z |
| format | Article |
| id | doaj.art-761f07692f58495eb7ab13732a3cb915 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T08:01:26Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-761f07692f58495eb7ab13732a3cb9152022-12-21T23:54:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5669710.1371/journal.pone.0056697Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L.Fangyuan ZhangXu LuZongyou LvLing ZhangMengmeng ZhuWeiming JiangGuofeng WangXiaofen SunKexuan TangThe phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned an ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly in leaf and flower. AaPYL9 protein can be localized in both nucleus and cytoplasm. Yeast two-hybrid assay shows AaPYL9 can specifically interact with AtABI1 but not with AtABI2, AtHAB1 or AtHAB2. ABA can enhance the interaction between AaPYL9 and AtABI1 while AaPYL9-89 Pro→Ser and AaPYL9-116 His→Ala point mutations abolishes the interaction. BiFC assay shows that AaPYL9 interacts with AtABI1 in nucleus in planta. Transgenic Arabidopsis plants over-expressing AaPYL9 are more sensitive to ABA in the seed germination and primary root growth than wild type. Consistent with this, ABA report genes have higher expression in AaPYL9 overexpressing plants compared to wild type after ABA treatment. Moreover, overexpression of AaPYL9 in A. annua increases not only drought tolerance, but also artemisinin content after ABA treatment, with significant enhancement of the expression of key genes in artemisinin biosynthesis. This study provides a way to develop A. annua with high-yielding artemisinin and high drought resistance.http://europepmc.org/articles/PMC3577733?pdf=render |
| spellingShingle | Fangyuan Zhang Xu Lu Zongyou Lv Ling Zhang Mengmeng Zhu Weiming Jiang Guofeng Wang Xiaofen Sun Kexuan Tang Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L. PLoS ONE |
| title | Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L. |
| title_full | Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L. |
| title_fullStr | Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L. |
| title_full_unstemmed | Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L. |
| title_short | Overexpression of the Artemisia orthologue of ABA receptor, AaPYL9, enhances ABA sensitivity and improves artemisinin content in Artemisia annua L. |
| title_sort | overexpression of the artemisia orthologue of aba receptor aapyl9 enhances aba sensitivity and improves artemisinin content in artemisia annua l |
| url | http://europepmc.org/articles/PMC3577733?pdf=render |
| work_keys_str_mv | AT fangyuanzhang overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT xulu overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT zongyoulv overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT lingzhang overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT mengmengzhu overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT weimingjiang overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT guofengwang overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT xiaofensun overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual AT kexuantang overexpressionoftheartemisiaorthologueofabareceptoraapyl9enhancesabasensitivityandimprovesartemisinincontentinartemisiaannual |