Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models
The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main subclasses in the SC lipid matrix are ceramides (CER), cholesterol, and free fatty acids. In inflammatory skin diseases, such as atopic dermatitis and psoriasi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227523000731 |
| _version_ | 1797737355669405696 |
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| author | Andreea Nădăban Jannik Rousel Dounia El Yachioui Gerrit S. Gooris Charlotte M. Beddoes Robert M. Dalgliesh Marc Malfois Robert Rissmann Joke A. Bouwstra |
| author_facet | Andreea Nădăban Jannik Rousel Dounia El Yachioui Gerrit S. Gooris Charlotte M. Beddoes Robert M. Dalgliesh Marc Malfois Robert Rissmann Joke A. Bouwstra |
| author_sort | Andreea Nădăban |
| collection | DOAJ |
| description | The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main subclasses in the SC lipid matrix are ceramides (CER), cholesterol, and free fatty acids. In inflammatory skin diseases, such as atopic dermatitis and psoriasis, the SC lipid composition is modulated compared to the composition in healthy SC. One of the main alterations is the molar ratio between the concentration of CER N-(tetracosanoyl)-sphingosine (CER NS) and CER N-(tetracosanoyl)-phytosphingosine (CER NP), which correlated with an impaired skin barrier function. In the present study, we investigated the impact of varying the CER NS:CER NP ratios on the lipid organization, lipid arrangement, and barrier functionality in SC lipid model systems. The results indicate that a higher CER NS:CER NP ratio as observed in diseased skin did not alter the lipid organization or lipid arrangement in the long periodicity phase encountered in SC. The trans-epidermal water loss, an indication of the barrier functionality, was significantly higher for the CER NS:CER NP 2:1 model (mimicking the ratio in inflammatory skin diseases) compared to the CER NS:CER NP 1:2 ratio (in healthy skin). These findings provide a more detailed insight into the lipid organization in both healthy and diseased skin and suggest that in vivo the molar ratio between CER NS:CER NP contributes to barrier impairment as well but might not be the main factor. |
| first_indexed | 2024-03-12T13:27:30Z |
| format | Article |
| id | doaj.art-76325d43d081451c8bc876cc2b41ec5b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-03-12T13:27:30Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-76325d43d081451c8bc876cc2b41ec5b2023-08-25T04:23:31ZengElsevierJournal of Lipid Research0022-22752023-08-01648100400Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid modelsAndreea Nădăban0Jannik Rousel1Dounia El Yachioui2Gerrit S. Gooris3Charlotte M. Beddoes4Robert M. Dalgliesh5Marc Malfois6Robert Rissmann7Joke A. Bouwstra8Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The NetherlandsDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands; Centre for Human Drug Research, Leiden, The NetherlandsDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The NetherlandsDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The NetherlandsDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The NetherlandsISIS Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Didcot, United KingdomALBA Synchrotron, Barcelona, SpainDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands; Centre for Human Drug Research, Leiden, The Netherlands; Leiden University Medical Center, Leiden, The NetherlandsDivision of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands; For correspondence: Joke A. BouwstraThe lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the skin barrier function. The three main subclasses in the SC lipid matrix are ceramides (CER), cholesterol, and free fatty acids. In inflammatory skin diseases, such as atopic dermatitis and psoriasis, the SC lipid composition is modulated compared to the composition in healthy SC. One of the main alterations is the molar ratio between the concentration of CER N-(tetracosanoyl)-sphingosine (CER NS) and CER N-(tetracosanoyl)-phytosphingosine (CER NP), which correlated with an impaired skin barrier function. In the present study, we investigated the impact of varying the CER NS:CER NP ratios on the lipid organization, lipid arrangement, and barrier functionality in SC lipid model systems. The results indicate that a higher CER NS:CER NP ratio as observed in diseased skin did not alter the lipid organization or lipid arrangement in the long periodicity phase encountered in SC. The trans-epidermal water loss, an indication of the barrier functionality, was significantly higher for the CER NS:CER NP 2:1 model (mimicking the ratio in inflammatory skin diseases) compared to the CER NS:CER NP 1:2 ratio (in healthy skin). These findings provide a more detailed insight into the lipid organization in both healthy and diseased skin and suggest that in vivo the molar ratio between CER NS:CER NP contributes to barrier impairment as well but might not be the main factor.http://www.sciencedirect.com/science/article/pii/S0022227523000731skinlipidsceramidessphingolipidscholesterolstratum corneum |
| spellingShingle | Andreea Nădăban Jannik Rousel Dounia El Yachioui Gerrit S. Gooris Charlotte M. Beddoes Robert M. Dalgliesh Marc Malfois Robert Rissmann Joke A. Bouwstra Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models Journal of Lipid Research skin lipids ceramides sphingolipids cholesterol stratum corneum |
| title | Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models |
| title_full | Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models |
| title_fullStr | Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models |
| title_full_unstemmed | Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models |
| title_short | Effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models |
| title_sort | effect of sphingosine and phytosphingosine ceramide ratio on lipid arrangement and barrier function in skin lipid models |
| topic | skin lipids ceramides sphingolipids cholesterol stratum corneum |
| url | http://www.sciencedirect.com/science/article/pii/S0022227523000731 |
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