Protocol for the isolation of mouse senescence-associated CD4+ T cells using flow cytometry and functional assays

Summary: Senescence-associated (SA) CD4+ T cells, which increase with age, may underlie the development of autoimmunity and chronic inflammation, but their pathological function remains understudied. Here, we present a protocol to isolate CD153+ SA-T cells and evaluate their characteristic responses...

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Bibliographic Details
Main Authors: Yuji Fukushima, Nagahiro Minato, Masakazu Hattori
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004392