Improvement of the Genome Editing Tools Based on 5FC/5FU Counter Selection in <i>Clostridium acetobutylicum</i>
Several genetic tools have been developed for genome engineering in <i>Clostridium acetobutylicum</i> utilizing 5-fluorouracil (5FU) or 5-fluorocytosine (5FC) resistance as a selection method. In our group, a method based on the integration, by single crossing over, of a suicide plasmid...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/11/2696 |