Protein fishing from single live cells

Abstract Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytop...

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Bibliographic Details
Main Authors: Elaheh Shekaramiz, Rupak Doshi, H. Kumar Wickramasinghe
Format: Article
Language:English
Published: BMC 2018-09-01
Series:Journal of Nanobiotechnology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12951-018-0395-5