Protein fishing from single live cells
Abstract Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytop...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-09-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-018-0395-5 |
| _version_ | 1828354348836454400 |
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| author | Elaheh Shekaramiz Rupak Doshi H. Kumar Wickramasinghe |
| author_facet | Elaheh Shekaramiz Rupak Doshi H. Kumar Wickramasinghe |
| author_sort | Elaheh Shekaramiz |
| collection | DOAJ |
| description | Abstract Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics. |
| first_indexed | 2024-04-14T02:23:05Z |
| format | Article |
| id | doaj.art-764507efa2fb44caa00d131b49e8d9d5 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-14T02:23:05Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-764507efa2fb44caa00d131b49e8d9d52022-12-22T02:17:58ZengBMCJournal of Nanobiotechnology1477-31552018-09-011611610.1186/s12951-018-0395-5Protein fishing from single live cellsElaheh Shekaramiz0Rupak Doshi1H. Kumar Wickramasinghe2Department of Biomedical Engineering, University of California IrvineDepartment of Electrical Engineering, University of California IrvineDepartment of Biomedical Engineering, University of California IrvineAbstract Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics.http://link.springer.com/article/10.1186/s12951-018-0395-5ProteomicsNanopipetteSingle cell analysisLive cell protein detection |
| spellingShingle | Elaheh Shekaramiz Rupak Doshi H. Kumar Wickramasinghe Protein fishing from single live cells Journal of Nanobiotechnology Proteomics Nanopipette Single cell analysis Live cell protein detection |
| title | Protein fishing from single live cells |
| title_full | Protein fishing from single live cells |
| title_fullStr | Protein fishing from single live cells |
| title_full_unstemmed | Protein fishing from single live cells |
| title_short | Protein fishing from single live cells |
| title_sort | protein fishing from single live cells |
| topic | Proteomics Nanopipette Single cell analysis Live cell protein detection |
| url | http://link.springer.com/article/10.1186/s12951-018-0395-5 |
| work_keys_str_mv | AT elahehshekaramiz proteinfishingfromsinglelivecells AT rupakdoshi proteinfishingfromsinglelivecells AT hkumarwickramasinghe proteinfishingfromsinglelivecells |