Protein fishing from single live cells
Abstract Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytop...
Main Authors: | Elaheh Shekaramiz, Rupak Doshi, H. Kumar Wickramasinghe |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-09-01
|
Series: | Journal of Nanobiotechnology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12951-018-0395-5 |
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