Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)

The total pentose quantity in buckwheat flour of cvs. Siva, Darja, Petra and Bednja 4n was determined using the volumetric bromide-bromate method. The Molisch test was used to determine the presence of carbohydrates in hydrolysates (72% H2SO4) and, in the same samples,  high-performance  liquid  ch...

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Main Author: Zlata LUTHAR
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 1998-09-01
Series:Acta Agriculturae Slovenica
Subjects:
Online Access:https://journals.uni-lj.si/aas/article/view/16036
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author Zlata LUTHAR
author_facet Zlata LUTHAR
author_sort Zlata LUTHAR
collection DOAJ
description The total pentose quantity in buckwheat flour of cvs. Siva, Darja, Petra and Bednja 4n was determined using the volumetric bromide-bromate method. The Molisch test was used to determine the presence of carbohydrates in hydrolysates (72% H2SO4) and, in the same samples,  high-performance  liquid  chromatography  (HPLC) - based  on  ion-exchange chromatography and after-column reaction derivation with copper 2,2’-bicinchoninate and detected by using a UV-VIS spectrophotometer - was used to determine monosaccharides glucose (hexose) and xylose (pentose). We established that the only pentose present in buckwheat flour is xylose, whose content in different analysed cultivars ranged from 5.44 to 6.51%.
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spelling doaj.art-76568b8e247d45279f2f0028c08d96512023-12-18T22:15:23ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-09-01711Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)Zlata LUTHAR0University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana The total pentose quantity in buckwheat flour of cvs. Siva, Darja, Petra and Bednja 4n was determined using the volumetric bromide-bromate method. The Molisch test was used to determine the presence of carbohydrates in hydrolysates (72% H2SO4) and, in the same samples,  high-performance  liquid  chromatography  (HPLC) - based  on  ion-exchange chromatography and after-column reaction derivation with copper 2,2’-bicinchoninate and detected by using a UV-VIS spectrophotometer - was used to determine monosaccharides glucose (hexose) and xylose (pentose). We established that the only pentose present in buckwheat flour is xylose, whose content in different analysed cultivars ranged from 5.44 to 6.51%. https://journals.uni-lj.si/aas/article/view/16036buckwheatflourcarbohydratepentosexylosehexose
spellingShingle Zlata LUTHAR
Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)
Acta Agriculturae Slovenica
buckwheat
flour
carbohydrate
pentose
xylose
hexose
title Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)
title_full Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)
title_fullStr Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)
title_full_unstemmed Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)
title_short Content and composition of pentose in buckwheat flour (Fagopyrum esculentum Moench)
title_sort content and composition of pentose in buckwheat flour fagopyrum esculentum moench
topic buckwheat
flour
carbohydrate
pentose
xylose
hexose
url https://journals.uni-lj.si/aas/article/view/16036
work_keys_str_mv AT zlataluthar contentandcompositionofpentoseinbuckwheatflourfagopyrumesculentummoench