Purification, crystallization and preliminary X-ray crystallographic analysis of human ppGpp hydrolase, Mesh1
Bacterial SpoT is a Mn2+-dependent pyrophosphohydrolase to hydrolyze guanosine 3’-diphosphate-5’-diphosphate to guanosine diphosphate and pyrophosphate. In this study, SpoT ortholog from Homo sapiens (hMesh1), was over-expressed in Escherichia coli, purified and crystallized using hanging-drop vapou...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2010-09-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | http://www.jastmag.org/journal/view.php?number=23 |