Purification of phospholipase B from Penicillium notatum by hydrophobic chromatography on palmitoyl cellulose.
Phospholipase B (lysolecithin acyl-hydrolase, EC 3.1.1.5) from the mycelia of Penicillium notatum (Institute for Fermentation, Osaka, Japan; No.4640) was adsorbed from a crude solution to palmitoyl cellulose. Adsorption was efficient at pH 4 at low ionic strength (10 mM buffer), and at pH 4-9 at hig...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1980-02-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520398230 |