Biosensors for the Detection of Food Pathogens
Food pathogens frequently cause foodborne diseases. There is a need to rapidly identify the source of the bacteria in order to contain their spread and epidemics. A pre-enrichment culture or a direct culture on agar plate are standard microbiological methods. In this review, we present an update o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-09-01
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| Series: | Foods |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2304-8158/3/3/511 |
| _version_ | 1811269205944696832 |
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| author | Palmiro Poltronieri Valeria Mezzolla Elisabetta Primiceri Giuseppe Maruccio |
| author_facet | Palmiro Poltronieri Valeria Mezzolla Elisabetta Primiceri Giuseppe Maruccio |
| author_sort | Palmiro Poltronieri |
| collection | DOAJ |
| description | Food pathogens frequently cause foodborne diseases. There is a need to rapidly identify the source of the bacteria in order to contain their spread and epidemics. A pre-enrichment culture or a direct culture on agar plate are standard microbiological methods. In this review, we present an update on alternative molecular methods to nucleic acid-based detection for species identification. Biosensor-based methods rely on the recognition of antigen targets or receptors by antibodies, aptamers or high-affinity ligands. The captured antigens may be then directly or indirectly detected through an antibody or high-affinity and high-specificity recognition molecule. Various different detection methods are discussed, from label-free sensors and immunosensors to fluorescence-based ones. Each method shows advantages and disadvantages in terms of equipment, sensitivity, simplicity and cost-effectiveness. Finally, lab-on-a-chip (LOC) devices are introduced briefly, with the potential to be fast, sensitive and useful for on-site bacteria detection in food processing laboratories to check potential contamination by sample monitoring combined with a rapid pre-enrichment step. |
| first_indexed | 2024-04-12T21:36:43Z |
| format | Article |
| id | doaj.art-769eb6c93dc54645a457f658c3164930 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-12T21:36:43Z |
| publishDate | 2014-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-769eb6c93dc54645a457f658c31649302022-12-22T03:15:52ZengMDPI AGFoods2304-81582014-09-013351152610.3390/foods3030511foods3030511Biosensors for the Detection of Food PathogensPalmiro Poltronieri0Valeria Mezzolla1Elisabetta Primiceri2Giuseppe Maruccio3Institute of Sciences of Food Productions, National Research Council, ISPA-CNR, Via Lecce-Monteroni, 73100 Lecce, ItalyInstitute of Sciences of Food Productions, National Research Council, ISPA-CNR, Via Lecce-Monteroni, 73100 Lecce, ItalyNNL, Institute of Nanoscience-CNR, Via per Arnesano, I-73100 Lecce, ItalyNNL, Institute of Nanoscience-CNR, Via per Arnesano, I-73100 Lecce, ItalyFood pathogens frequently cause foodborne diseases. There is a need to rapidly identify the source of the bacteria in order to contain their spread and epidemics. A pre-enrichment culture or a direct culture on agar plate are standard microbiological methods. In this review, we present an update on alternative molecular methods to nucleic acid-based detection for species identification. Biosensor-based methods rely on the recognition of antigen targets or receptors by antibodies, aptamers or high-affinity ligands. The captured antigens may be then directly or indirectly detected through an antibody or high-affinity and high-specificity recognition molecule. Various different detection methods are discussed, from label-free sensors and immunosensors to fluorescence-based ones. Each method shows advantages and disadvantages in terms of equipment, sensitivity, simplicity and cost-effectiveness. Finally, lab-on-a-chip (LOC) devices are introduced briefly, with the potential to be fast, sensitive and useful for on-site bacteria detection in food processing laboratories to check potential contamination by sample monitoring combined with a rapid pre-enrichment step.http://www.mdpi.com/2304-8158/3/3/511food pathogensrapid identificationmolecular recognitiondetection methodslabellingantibody chipslab-on-a-chip |
| spellingShingle | Palmiro Poltronieri Valeria Mezzolla Elisabetta Primiceri Giuseppe Maruccio Biosensors for the Detection of Food Pathogens Foods food pathogens rapid identification molecular recognition detection methods labelling antibody chips lab-on-a-chip |
| title | Biosensors for the Detection of Food Pathogens |
| title_full | Biosensors for the Detection of Food Pathogens |
| title_fullStr | Biosensors for the Detection of Food Pathogens |
| title_full_unstemmed | Biosensors for the Detection of Food Pathogens |
| title_short | Biosensors for the Detection of Food Pathogens |
| title_sort | biosensors for the detection of food pathogens |
| topic | food pathogens rapid identification molecular recognition detection methods labelling antibody chips lab-on-a-chip |
| url | http://www.mdpi.com/2304-8158/3/3/511 |
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