Quantitative RT-PCR Amplification of RNA in Single Mouse Oocytes and Preimplantation Embryos
We describe a simple whole-cell method for quantitative reverse transcription (RT) PCR amplification of RNA that consistently allows the analysis of trace amounts of RNA, such as those carried by a fraction of a single mouse oocyte or preimplantation embryo, without organic extraction. The method is...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98244st02 |