Quantifying Trade-Offs in the Choice of Ribosomal Barcoding Markers for Fungal Amplicon Sequencing: a Case Study on the Grapevine Trunk Mycobiome

Quantifying Trade-Offs in the Choice of Ribosomal Barcoding Markers for Fungal Amplicon Sequencing: a Case Study on the Grapevine Trunk Mycobiome

ABSTRACT The evolution of sequencing technology and multiplexing has rapidly expanded our ability to characterize fungal diversity in the environment. However, obtaining an unbiased assessment of the fungal community using ribosomal markers remains challenging. Longer amplicons were shown to improve...

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Bibliographic Details
Main Authors: Vinciane Monod, Valérie Hofstetter, Vivian Zufferey, Olivier Viret, Katia Gindro, Daniel Croll
Format: Article
Language:English
Published: American Society for Microbiology 2022-12-01
Series:Microbiology Spectrum
Subjects:
amplicon sequencing
grapevine
fungi
internal transcribed spacer
mycobiome
barcoding
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02513-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.02513-22

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