Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking

Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking

Here we provide demonstration that fast fluorescence fluctuation spectroscopy is a fast and robust approach to extract information on the dynamics of molecules enclosed within subcellular nanostructures (e.g., organelles or vesicles) which are also moving in the complex cellular environment. In more...

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Bibliographic Details
Main Authors: Filippo Begarani, Francesca D’Autilia, Gianmarco Ferri, Luca Pesce, Fabio Azzarello, Valentina De Lorenzi, William Durso, Ambra Del Grosso, Marco Cecchini, Francesco Cardarelli
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:International Journal of Molecular Sciences
Subjects:
diffusion
correlation spectroscopy
fluorescence
living cells
subcellular scale
nanoscale
Online Access:https://www.mdpi.com/1422-0067/23/14/7623

Internet

https://www.mdpi.com/1422-0067/23/14/7623

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