Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

Abstract Background Transposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid. However, many bacterial species cannot be efficiently conjugated or transformed...

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Bibliographic Details
Main Authors: Santa S. Naorem, Jin Han, Stephanie Y. Zhang, Junyi Zhang, Lindsey B. Graham, Angelou Song, Cameron V. Smith, Fariha Rashid, Huatao Guo
Format: Article
Language:English
Published: BMC 2018-10-01
Series:BMC Microbiology
Subjects:
Transposon mutagenesis
IPTG
Conditional suicide plasmid
Escherichia coli
Pseudomonas
Acinetobacter
Online Access:http://link.springer.com/article/10.1186/s12866-018-1319-0

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http://link.springer.com/article/10.1186/s12866-018-1319-0

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