In-Cell Labeling Coupled to Direct Analysis of Extracellular Vesicles in the Conditioned Medium to Study Extracellular Vesicles Secretion with Minimum Sample Processing and Particle Loss

In-Cell Labeling Coupled to Direct Analysis of Extracellular Vesicles in the Conditioned Medium to Study Extracellular Vesicles Secretion with Minimum Sample Processing and Particle Loss

Extracellular vesicles (EVs) are involved in a multitude of physiological functions and play important roles in health and disease. The largest proportion of studies on EVs is based on the analysis and characterization of EVs secreted in the cell culture medium. These studies remain challenging due...

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Bibliographic Details
Main Authors: Anissa Viveiros, Vaibhavi Kadam, John Monyror, Luis Carlos Morales, Desmond Pink, Aja M. Rieger, Simonetta Sipione, Elena Posse de Chaves
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Cells
Subjects:
extracellular vesicles
fluorescent labeling
imaging flow cytometry
ultracentrifugation
size exclusion chromatography
nanoparticle tracking analysis
Online Access:https://www.mdpi.com/2073-4409/11/3/351

Internet

https://www.mdpi.com/2073-4409/11/3/351

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