Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis

Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis

Abstract Background Precise gene targeting (GT) is a powerful tool for heritable precision genome engineering, enabling knock-in or replacement of the endogenous sequence via homologous recombination. We recently established a CRISPR/Cas9-mediated approach for heritable GT in Arabidopsis thaliana (A...

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Bibliographic Details
Main Authors: Jing Li, Dali Kong, Yongping Ke, Wenjie Zeng, Daisuke Miki
Format: Article
Language:English
Published: BMC 2024-01-01
Series:BMC Biology
Subjects:
Genome engineering
Gene targeting
CRISPR/Cas9
Multiple sgRNAs
Arabidopsis
Online Access:https://doi.org/10.1186/s12915-024-01810-7

Internet

https://doi.org/10.1186/s12915-024-01810-7

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