Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

Abstract Background The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair...

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Bibliographic Details
Main Authors: Kun Zhang, Xingpeng Duan, Peng Cai, Linhui Gao, Xiaoyan Wu, Lun Yao, Yongjin J. Zhou
Format: Article
Language:English
Published: BMC 2022-09-01
Series:Microbial Cell Factories
Subjects:
Pichia pastoris
CRISPR/Cas9
Gene editing
Homology-directed repair
Metabolic engineering
Online Access:https://doi.org/10.1186/s12934-022-01908-z

Internet

https://doi.org/10.1186/s12934-022-01908-z

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