Investigation of Different Library Preparation and Tissue of Origin Deconvolution Methods for Urine and Plasma cfDNA Methylome Analysis

Investigation of Different Library Preparation and Tissue of Origin Deconvolution Methods for Urine and Plasma cfDNA Methylome Analysis

Methylation sequencing is a promising approach to infer the tissue of origin of cell-free DNA (cfDNA). In this study, a single- and a double-stranded library preparation approach were evaluated with respect to their technical biases when applied on cfDNA from plasma and urine. Additionally, tissue o...

Full description

Bibliographic Details
Main Authors: Nicholas Kueng, Daniel Sidler, Vanessa Banz, Carlo R. Largiadèr, Charlotte K. Y. Ng, Ursula Amstutz
Format: Article
Language:English
Published: MDPI AG 2023-07-01
Series:Diagnostics
Subjects:
cfDNA
methylation sequencing
tissue of origin
enzymatic conversion
transplant
urine
Online Access:https://www.mdpi.com/2075-4418/13/15/2505

Internet

https://www.mdpi.com/2075-4418/13/15/2505

Similar Items