Effective Expression of the Serratia marcescens Phospholipase A1 Gene in Escherichia coli BL21(DE3), Enzyme Characterization, and Crude Rapeseed Oil Degumming via a Free Enzyme Approach

Effective Expression of the Serratia marcescens Phospholipase A1 Gene in Escherichia coli BL21(DE3), Enzyme Characterization, and Crude Rapeseed Oil Degumming via a Free Enzyme Approach

Crude oil degumming by phospholipid removal is crucial to guarantee oil quality. Phospholipase degumming could produce green vegetable oil by reducing energy consumption and protecting the environment. To develop a novel phospholipase for oil degumming, we cloned the Serratia marcescens outer membra...

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Bibliographic Details
Main Authors: Peizhou Yang, Yun Wu, Suwei Jiang, Zhi Zheng, Zhigang Hou, Dongdong Mu, Wei Xiao, Shaotong Jiang, Yung-Hun Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Serratia marcescens
phospholipase A
enzymatic degumming
rapeseed oil
enzyme characterization
Lecitase Ultra
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2019.00272/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2019.00272/full

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