Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values
Overexpression of Glu-1Bx7 via allele 1Bx7<sup>OE</sup> significantly contributes to high dough strength in some wheat varieties and is useful for improving wheat quality. However, the proportion of wheat varieties containing Bx7<sup>OE</sup> is quite low. In this study, four...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1244 |
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| author | Myoung-Hui Lee Changhyun Choi Kyeong-Hoon Kim Jae-Han Son Go-Eun Lee Jun-Yong Choi Chon-Sik Kang Jiyoung Shon Jong-Min Ko Kyeong-Min Kim |
| author_facet | Myoung-Hui Lee Changhyun Choi Kyeong-Hoon Kim Jae-Han Son Go-Eun Lee Jun-Yong Choi Chon-Sik Kang Jiyoung Shon Jong-Min Ko Kyeong-Min Kim |
| author_sort | Myoung-Hui Lee |
| collection | DOAJ |
| description | Overexpression of Glu-1Bx7 via allele 1Bx7<sup>OE</sup> significantly contributes to high dough strength in some wheat varieties and is useful for improving wheat quality. However, the proportion of wheat varieties containing Bx7<sup>OE</sup> is quite low. In this study, four cultivars containing 1Bx7<sup>OE</sup> were selected, and among the selected varieties, Chisholm (1Ax2*, 1Bx7<sup>OE</sup> + 1By8*, and 1Dx5 + 1Dx10) was crossed with Keumkang, a wheat variety that contains 1Bx7 (1Ax2*, 1Bx7 + 1By8, and 1Dx5 + 1Dx10). SDS-PAGE and UPLC analyses showed that the expression of the high-molecular-weight glutenin subunit (HMW-GS) 1Bx7 was significantly higher in NILs (1Ax2*, 1Bx7<sup>OE</sup> + 1By8*, and 1Dx5 + 1Dx10) compared with that in Keumkang. Wheat quality was analyzed with near infrared reflectance spectroscopy by measuring the protein content and SDS-sedimentation of NILs. The protein content of NILs (12.94%) was 21.65% higher than that of Chisholm (10.63%) and 4.54% higher than that of Keumkang (12.37%). In addition, the SDS-sedimentation value of NILs (44.29 mL) was 14.97% and 16.44% higher than that of Keumkang (38.52 mL) and Chisholm (38.03 mL), respectively. This study predicts that the quality of domestic wheat can be improved by crossbreeding with 1Bx7<sup>OE</sup>-containing cultivars. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T06:00:17Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-779fe1bc0e1e4c15861f40e5aad4f2152023-11-17T13:21:13ZengMDPI AGPlants2223-77472023-03-01126124410.3390/plants12061244Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation ValuesMyoung-Hui Lee0Changhyun Choi1Kyeong-Hoon Kim2Jae-Han Son3Go-Eun Lee4Jun-Yong Choi5Chon-Sik Kang6Jiyoung Shon7Jong-Min Ko8Kyeong-Min Kim9Wheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaWheat Research Team, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of KoreaOverexpression of Glu-1Bx7 via allele 1Bx7<sup>OE</sup> significantly contributes to high dough strength in some wheat varieties and is useful for improving wheat quality. However, the proportion of wheat varieties containing Bx7<sup>OE</sup> is quite low. In this study, four cultivars containing 1Bx7<sup>OE</sup> were selected, and among the selected varieties, Chisholm (1Ax2*, 1Bx7<sup>OE</sup> + 1By8*, and 1Dx5 + 1Dx10) was crossed with Keumkang, a wheat variety that contains 1Bx7 (1Ax2*, 1Bx7 + 1By8, and 1Dx5 + 1Dx10). SDS-PAGE and UPLC analyses showed that the expression of the high-molecular-weight glutenin subunit (HMW-GS) 1Bx7 was significantly higher in NILs (1Ax2*, 1Bx7<sup>OE</sup> + 1By8*, and 1Dx5 + 1Dx10) compared with that in Keumkang. Wheat quality was analyzed with near infrared reflectance spectroscopy by measuring the protein content and SDS-sedimentation of NILs. The protein content of NILs (12.94%) was 21.65% higher than that of Chisholm (10.63%) and 4.54% higher than that of Keumkang (12.37%). In addition, the SDS-sedimentation value of NILs (44.29 mL) was 14.97% and 16.44% higher than that of Keumkang (38.52 mL) and Chisholm (38.03 mL), respectively. This study predicts that the quality of domestic wheat can be improved by crossbreeding with 1Bx7<sup>OE</sup>-containing cultivars.https://www.mdpi.com/2223-7747/12/6/1244hexaploid wheat1Bx7<sup>OE</sup>1Bx7 promoterultra-performance liquid chromatographynear infrared reflectance spectroscopy |
| spellingShingle | Myoung-Hui Lee Changhyun Choi Kyeong-Hoon Kim Jae-Han Son Go-Eun Lee Jun-Yong Choi Chon-Sik Kang Jiyoung Shon Jong-Min Ko Kyeong-Min Kim Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values Plants hexaploid wheat 1Bx7<sup>OE</sup> 1Bx7 promoter ultra-performance liquid chromatography near infrared reflectance spectroscopy |
| title | Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values |
| title_full | Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values |
| title_fullStr | Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values |
| title_full_unstemmed | Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values |
| title_short | Generation of Wheat Near-Isogenic Lines Overexpressing 1Bx7 Glutenin with Increased Protein Contents and SDS-Sedimentation Values |
| title_sort | generation of wheat near isogenic lines overexpressing 1bx7 glutenin with increased protein contents and sds sedimentation values |
| topic | hexaploid wheat 1Bx7<sup>OE</sup> 1Bx7 promoter ultra-performance liquid chromatography near infrared reflectance spectroscopy |
| url | https://www.mdpi.com/2223-7747/12/6/1244 |
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